Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-producing Conditions

LI Tingting1,2, CHEN Qian2, SHI Ping1, GENG Shu3,†

ACTA Scientiarum Naturalium Universitatis Pekinensis - - News -

1. Shenzhen Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055; 2. Beijing Engineering Research Center for Advanced Wastewater Treatment, Department of Environmental Engineering , Peking University, Beijing 100871; 3. Sino-us Joint Food Safety Research Center, Northwest A&F University, Yangling 712100; † Corresponding author, E-mail: sgeng@ucdavis.edu

Abstract 53#, a strain of efficient alginate degrading bacteria, is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified as Paenibacillus agaridevorans based on physiological characteristics and 16S RRNA sequencing. The optimaltion yield condition of strain 53# is identified at ph=8, T=25ºC, Nacl 15 g/l, and sodium alginate 15 g/l by orthogonal experiment and analysis, and the highest enzyme activity is 390.53±17.32 U/ML. Strain 53# has the advantages such as being cultivated easily, producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae. Key words alginate degradation; alginate lyase; Paenibacillus; biofuel

近年来, 海洋藻类以生长速度快, 光合作用效率高, 不含难降解木质素, 不与粮食作物争夺土地和淡水资源等优点成为第三代生物能源原料[1]。海洋藻类分为微藻和大型海藻。由于油脂含量较高,微藻仅适用于生产生物柴油; 因碳水化合物含量较高, 大型海藻被认为是开发生物乙醇的优质原料。

以大型海藻中典型的褐藻门海带为例, 干重的30%~40%为碳水化合物[2], 这些碳水化合物以褐藻胶和甘露醇为主, 其中褐藻胶占碳水化合物总量的67%以上[3]。

[4] Horn 等 最早将褐藻门海带用于生物乙醇的发酵, 发现毕赤酵母 Pichia angophorae 可以利用北

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