Isolation of Fungal Strains in Soil of Selenium Mining Area and Screening for Their Anti-tumor Activity

RAN Xiling1, LIU Yu1, TIAN Congkui1,†, ZHU Tingting2, SONG Yanan1, MO Yuexian1

ACTA Scientiarum Naturalium Universitatis Pekinensis - - News -

1. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province & Wuling Mountain Institute of Natural Medicine, Hubei University for Nationalities, Enshi 445000; 2. State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology & Shenzhen Academy of Environmental Sciences, Shenzhen 518001; † Corresponding author, E-mail:

Abstract The fungal strains were obtained by single colony isolation technique from the soil of the world’s unique proved independent selenium deposit. The anti-tumor activity was assayed by MTT method using Mdamb-231cells, Hela cells and SMMC-7721 cells, respectively, together with morphological observation of these cells under inversed light microscope. The 73 terrestrial fungal strains were isolated from the soil samples collected from 6 different areas in Enshi area of Hubei Province, China. Among them, 32 strains produced fermentation products obviously inhibiting the Hela cells, 13 strains inhibiting the MDA-MB-231 cells and 9 strains inhibiting the SMMC-7721 cells with IC50 value less than 90 µg/ml. These antitumor-metabolite-producing strains have provided fungal strains for further studies on the bioactive metabolites. Key words fungi; selenium mining area; terrestrial fungus; isolation and cultivation; anti-tumor activity

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