不同产地杜仲叶红外光谱与薄层色谱特征研究

CJI (Traditional Chinese Medicine) - - Chinese Journal Of Information On Traditional Chin -

1 2 3邢亚楠 ,阿来•赛坎 ,田树革

1.新疆师范大学化学化工学院,新疆 乌鲁木齐 830054;2.新疆医科大学中医学院,新疆 乌鲁木齐, 830011;

3.新疆医科大学中心实验室,新疆 乌鲁木齐 830011

摘要:目的 分析比较不同产地杜仲叶的红外光谱,建立杜仲叶的薄层色谱鉴别方法,为其质量控制提供依据。方法 利用红外光谱、二阶导数谱图对6种不同产地杜仲叶进行特征峰的指认和对比分析,并进行聚类分析;通过薄层色谱对杜仲叶中活性成分进行分离和鉴别。结果 通过特征峰峰形、峰位与峰强的对比,发现不同产地杜仲叶之间存在差异,聚类结果显示可将6个产地杜仲叶分为1类;薄层色谱结果显示6批样品主斑点一致,但不同产地的样品间微量成分斑点存在差异。结论 2种方法直观、简单、方便、快速,为杜仲叶的分离鉴别和质量控制提供了依据。

关键词:杜仲叶;红外光谱;二阶导数谱图;聚类分析;薄层色谱

DOI: 10.3969/j.issn.1005-5304.2017.02.022

中图分类号: R284.1 文献标识码: A 文章编号: 1005-5304(2017)02-0088-03

Research on Characteristics of Eucommia ulmoides Leaf from Different Producing Areas by IR and TLC

XING Ya-nan1, Alai Saikan2, TIAN Shu-ge3 (1. College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi 830054, China; 2. College of TCM, Xinjiang Medical University, Urumqi 830011, China; 3. The Central Laboratory of Xinjiang Medical University, Urumqi 830011, China)

Abstract: Objective To analyze and compare the Eucommia ulmoides leaves from different producing areas by IR; To establish a TLC method for identification of Eucommia ulmoides leaves; To provide a reference for its quality control. Methods The characteristic absorption peaks were identified and the positions and intensities of Eucommia ulmoides leaves from 6 producing areas were compared by IR and second derivative spectrum, including clustering analysis; Separation and identification of the active components in Eucommia ulmoides leaves were conducted by TLC. Results There a difference exited in the characteristic absorption peaks in peakshapes, positions and intensities. And the samples from 6 producing areas could be divided into one category. Results found 6 batches of sample spots of consistent by TLC, but between samples of different producing areas trace components spots existed differences. Conclusion The two methods are direct, simple, fast and convenient, which can provide a reliable evidence for identification and quality control of the Eucommia ulmoides leaves.

Key words: Eucommia ulmoides leaves; IR; second derivative spectrum; clustering analysis; TLC 杜仲叶是杜仲科杜仲属植物杜仲 Eucommia

[1] ulmoides Oliv.的干燥叶 。杜仲叶与皮的化学成分基本一致[ 2],且具有同等功效,在医药、保健、食品方面发展前景广阔。杜仲叶富含绿原酸、槲皮素、山柰酚、桃叶珊瑚苷、京尼平甘酸等活性物质及多种氨基酸、维生素[ 3],有良好的降压、利尿、保肝、抗肿瘤、抗菌、抗衰老和中枢镇静等多种药理作用。本研究采

[4]

用傅里叶变换红外光谱 、二阶导数红外光谱、聚类

[5-6] [7]

分析 与薄层色谱法 对不同产地的 6 种杜仲叶进行鉴别研究,为杜仲叶的鉴定和质量控制提供依据。 基金项目:新疆维吾尔自治区科技计划(201130105-5)

1 仪器与试药

TENSOR27 傅里叶变换近红外光谱仪(德国Bruker 公司) DTGS 检测器,光谱扫面范围 4000~

400 cm- 1,光谱分辨率2 cm- 1,累计扫描次数32 次。

769YP-15A粉末压片机(天津市科器高新技术公司);

70-1型远红外干燥箱(上海申光仪器仪表有限公司);薄层色谱点样器( Linomat5 CAMAG),薄层色谱点样针( CAMAG),薄层色谱照相仪( REPROSTAR 3),薄层色谱扫描仪( TLC SANNER3 CAMAG)

杜仲叶样品采自5个不同地区( S1 为 2014 年摘于新疆乌鲁木齐, S2 为 2015年摘于新疆乌鲁木齐,

S3为陕西铜州, S4为湖北襄阳, S5为湖南张家界,

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