参芪抑瘤方药物血清对胃癌MGC-803细胞增殖的影响

CJI (Traditional Chinese Medicine) - - News -

马春林1,吴红彦 1,2,3,李海龙 1,2,陈杰 1,张宣 1,李红亮 1

1.甘肃中医药大学,甘肃 兰州 730000;

2.甘肃省中医方药挖掘和创新转化重点实验室,甘肃 兰州 730000;

3.甘肃省中药新产品创制工程实验室,甘肃 兰州 730000

摘要:目的 观察参芪抑瘤方药物血清对胃癌MGC-803细胞增殖的影响,探讨其相关机制。方法 以参芪抑瘤方不同浓度药物血清干预胃癌 MGC-803 细胞后,MTT 法检测细胞增殖,流式细胞仪检测细胞周期, qRT-PCR 检测 CDKN1B、CDKN1C 基因表达,Western blot 检测 p27、p57 蛋白表达。结果 3%、5%、10%药物血清作用于 MGC-803 细胞 24、48、72 h,细胞增殖水平显著降低(P<0.01),且与时间和剂量呈依赖关系;

3%、5%、10%药物血清作用于 MGC-803 细胞 24 h 后,G0/G1 期细胞增多,S 期细胞减少;qRT-PCR 检测结果显示,与对照组和空白血清组比较,药物血清各剂量组MGC-803 细胞 CDKN1B、CDKN1C mRNA表达显

著上调(P<0.01);Western blot 检测结果显示,与对照组比较,药物血清各剂量组 MGC-803 细胞 p27、p57

蛋白表达显著上调(P<0.01)。结论 参芪抑瘤方药物血清可抑制胃癌 MGC-803 细胞的增殖,其机制可能是通过调节 CDKN1B、CDKN1C mRNA和蛋白表达,进而干预细胞周期。关键词:参芪抑瘤方;药物血清;胃癌细胞;细胞增殖;细胞周期

DOI:10.3969/j.issn.1005-5304.2017.03.013

中图分类号:R285.5 文献标识码:A 文章编号:1005-5304(2017)03-0053-04

Effects of Medicinal Serum of Shenqi Yiliu Formula on Proliferation of Gastric Cancer MGC-803 Cells

MA Chun-lin1, WU Hong-yan1,2,3, LI Hai-long1,2, CHEN Jie1, ZHANG Xuan1, LI Hong-liang1

(1. Gansu University of Chinese Medicine, Lanzhou 730000, China; 2. State Key Laboratory of Traditional Chinese Medicine Excavation Innovation and Transformation of Gansu Province, Lanzhou 730000, China; 3. Laboratory for TCM New Products Development Engineering of Gansu Province, Lanzhou 730000, China)

Abstract: Objective To investigate the inhibitory effects of medicinal serum of Shenqi Yiliu Formula on cell proliferation of gastric cancer MGC-803 cells; To discuss relevant mechanism. Methods After treated with different concentrations of medicinal serum of Shenqi Yiliu Formula, gastric cancer MGC-803 cells were tested by the following methods: MTT was employed to test the proliferation of gastric cancer MGC-803 cells; Flow cytometry was used to detect cell cycle; qRT-PCR was used to detect the genetic expressions of CDKN1B and CDKN1C; Western blot was employed to test the protein expressions of p27 and p57. Results When 3%, 5% and 10% medicinal serum of Shenqi Yiliu Formula was treated to MGC-803 cells for 24 h, 48 h and 72 h, proliferation of cells decreased significantly (P<0.01), with time- and dosage-dependent relationship. When 3%, 5% and 10% medicinal serum of Shenqi Yiliu Formula was treated to MGC-803 cells for 24 h, cells in G0/G1 increased, decreased in S. qRT-PCR results showed that compared with the blank control group and the negative control group, mRNA expressions of

CDKN1B and CDKN1C of MGC-803 cells in medicinal serum all dose group increased significantly (P<0.01). Western blot results showed that compared with the blank control group, protein expressions of p27 and p57 of

MGC-803 cells in medicinal serum all dose group increased significantly (P<0.01). Conclusion Medicinal serum of Shenqi Yiliu Formula can inhibit MGC-803 cells proliferation and the mechanism may be through adjusting CDKN1B,

CDKN1C mRNA and proteins expression to intervene in the cell cycle.

Key words: Shenqi Yiliu Formula; medicinal serum; gastric cancer cell; cell proliferation; cell cycle

基金项目:甘肃中医药大学研究生创新基金(2015CX11);兰州市人才创新创业扶持项目(2015-RC-24)

通讯作者:吴红彦,E-mail:wuhy@163.com

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