艾纳香妇科洗液处方筛选及抑菌试验

CJI (Traditional Chinese Medicine) - - News -

陈艳 1,2,3,王凯 2,3,杨全 1,庞玉新 2,3,4

1.广东药科大学,广东 广州 510006;

2.中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点实验室,海南 儋州 571737;

3.海南省艾纳香工程技术研究中心,海南 儋州 571737;4.中国中医科学院中药资源中心,北京 100700摘要:目的 对艾纳香妇科洗液进行处方筛选,并通过抑菌试验进行抑菌效果考察,为其临床应用提供依据。方法 以溶解等量艾粉所需3 种增溶剂[Tween-80、月桂醇聚氧乙烯醚硫酸钠(SLES)、氢化蓖麻油(RH-40)]的消耗量及溶液是否澄清透明为评价指标,筛选出最优增溶剂;使用滤纸片法,以抑菌圈大小为评价指标,从

3 种抑菌剂[2 g/L 杰马 BP、2 mL/L 聚六亚甲基双胍盐酸盐(PHMB)、2 mL/L A.SAP]中筛选对金黄色葡萄球菌、大肠埃希菌和白色念珠菌具有最佳抑菌效果的抑菌剂;以艾粉溶解百分比为评价指标,采用正交试验对洗液中辅料添加量进行筛选,得到最优处方。最后,对所得洗液进行体外抑菌试验,考察其抑菌效果及稳定性。结果 增溶效果为 RH-40>SLES>Tween-80;抑菌圈直径为 PHMB>A.SAP>杰马BP;筛选所得最优处方为:0.6 mg/mL

艾粉、0.04 g/mL SLES、0.01 g/mL CAB-35、0.2% A.SAP、10%丙二醇水溶液。艾纳香妇科洗液供试品的抑菌率均大于 90%;加速试验下放置 3 个月,供试品抑菌率均大于 80%。结论 艾纳香妇科洗液处方合理可行,工艺简单,材料易得,并具有较强的抑菌作用,且抑菌性能稳定性较好。关键词:艾粉;妇科洗液;处方筛选;抑菌试验

DOI:10.3969/j.issn.1005-5304.2017.03.021

中图分类号:R283.5 文献标识码:A 文章编号:1005-5304(2017)03-0087-04

Prescription Screening Test and Inhibitory Rate Experiment of Blumea Gynecological Lotion

CHEN Yan1,2,3, WANG Kai2,3, YANG Quan1, PANG Yu-xin2,3,4 (1. Guangdong Parmaceutical University, Guangzhou

510006, China; 2. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou 571737, China; 3. Hainan Provincial Engineering Resource Center for Blumea balsamifera, Danzhou 571737, China; 4. National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China)

Abstract: Objective To screen the presciption of Blumea gynecological lotion and investigate the inhibitory effect through inhibition rate test; To provide a basis for clinical application. Methods Consumption of Tween-80, SLES, hydrogenated castor oil (RH-40), and clarity of the solution were selected as the evaluation indexes to optimize the solubilizing agents. By using the filter paper method and the inhibition zone size as the evaluation index, the antimicrobial effect on Staphylococcus aureus. Escherichia coli and Candida albicans was studied to screen the best antibacterial agent from 2 g/L GM-BP, 2 mL/L PHMB, 2 mL/L A.SAP. With Blumeapowder dissolution percentage as the evaluation index, orthogonal test was used to screen additives in the lotion. Finally, the bacteriostasis effect and stability of the obtained lotion were investigated in vitro. Results The order of solubilizing effect was RH-40>

SLES>Tween-80, and that of inhibition zone size was PHMB>A.SAP>GM-BP. The best prescription as follows:

0.6 mg/mL Blumea powder, 0.04 g/mL SLES, 0.01 g/mL CAB-35, 10% propylene glycol. Bacteriostatic rates of Blumea gynecology lotion sample were more than 90%, and bacteriostatic rates remained more than 80% after 3 months. Conclusion The prescription of Blumea gynecology lotion is reasonable and practicable, with simple preparation process, available materials and good inhibitory effect and stability.

Key words: Blumea powder; gynecological lotion; prescription selection; bacteriostatic test

基金项目:国家自然科学基金(81374065);贵州省科技计划(黔科合重大专项字[2013]6011 号)

通讯作者:杨全,E-mail:yangquan7208@vip.163.com

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