电针对坐骨神经损伤大鼠脑源性神经营养因子的影响

CJI (Traditional Chinese Medicine) - - 中国中医药信息杂志 -

叶晓春,邵水金,国海东,韩小晶,刘玉璞,陆萍萍

上海中医药大学基础医学院,上海 201203

摘要:目的 观察电针对坐骨神经损伤(SNI)大鼠脑源性神经营养因子(BDNF)的影响,探讨其治疗SNI的生物学机制。方法 选取成年雄性 Wistar 大鼠 50只,分离并切断大鼠坐骨神经后于体式显微镜下将两侧断端拉入神经再生室内造模。大鼠随机分为正常组、假手术组、模型组和电针组。电针组进行电针治疗,连续 28 d。治疗结束后,HE染色观察神经再生,免疫荧光检测神经组织和脊髓BDNF 的表达,ELISA检测血清BDNF的表达。结果 电针组轴突再生数量明显多于模型组,且大体轮廓较清晰;与模型组比较,电针组可促进大鼠神经组织、脊髓和血清BDNF 的表达(P<0.01)。结论 电针可能通过上调神经损伤后BDNF的表达,促进大鼠 SNI的再生修复。

关键词:电针;坐骨神经损伤;脑源性神经营养因子;轴突;大鼠

DOI:10.3969/j.issn.1005-5304.2017.06.015

中图分类号:R245 文献标识码:A 文章编号:1005-5304(2017)06-0060-04

Effects of Electroacupuncture on Brain Derived Neurotrophic Factor of Rats with Sciatic Nerve Injury

YE Xiao-chun, SHAO Shui-jin, GUO Hai-dong, HAN Xiao-jing, LIU Yu-pu, LU Ping-ping (School of Basic Medicine, Shanghai University of TCM, Shanghai 201203, China)

Abstract: Objective To explore the effects of electroacupuncture on brain derived neurotrophic factor (BDNF) of rats with sciatic nerve injury (SNI); To discuss its biological mechanism for treatment of SNI. Methods Fifty adult male Wistar rats were chosen, and the sciatic nerves of rats were cut off and pulled on both sides of the cut ends into nerve regeneration chamber. The rats were randomly divided into normal group, sham-operation group, model group, and electroacupuncture group. In the electroacupuncture group, the rats were treated by electroacupuncture for 28 days. After the treatment, the nerve regeneration was observed through HE staining. Immunofluorescence was used to analyze the expression changes of BDNF in the nerve tissue and spinal cord. ELISA was used to observe the changes of expression of serum BDNF. Results The amount of axon regeneration in the electroacupuncture group was obviously more than that in the model group, and the outline of the tissue more clear. Electroacupuncture could promote the expression of BDNF in the nerve, spinal cord and serum of SNI of rats compared with model group (P<0.01). Conclusion Electroacupuncture can promote the repairment and regeneration of SNI in rats by upregulating the expression of BDNF.

Key words: electroacupuncture; sciatic nerve; brain derived neurotrophic factor; axon; rats

周围神经损伤(PNI)属中医学“伤筋”“痿证”范畴。《诸病源候论•金疮伤筋断骨候》云:“夫金疮始伤之时,半伤其筋,荣卫不通,其疮虽愈合后,仍令痹不仁也。”目前治疗方法有神经吻合技术、细胞移植及相关药物应用等,但神经功能的恢复仍不理

想[1-2]。实验和临床研究表明,电针在损伤神经的功能恢复方面疗效肯定和优势明显,能明显提高 PNI

患肢的肌力,促进神经功能的恢复,减轻后遗症[3-4]。

基金项目:国家自然科学基金(81373754)

通讯作者:邵水金,E-mail:shaoshuijin@163.com 本实验观察电针治疗后脑源性神经营养因子(brain derived neurotrophic factor,BDNF)在各部位的变化及其对坐骨神经损伤(SNI)大鼠 BDNF 的影响,探讨电针治疗 SNI的生物学机制。

1 实验材料

1.1 动物

SPF级成年雄性Wistar 大鼠50只,体质量(200±

20)g,上海中医药大学动物实验中心,动物许可证号 SYXK(沪)2014-0008。饲养于上海中医药大学SPF级动物实验室,温度22~25 ℃,相对湿度40%~

70%,每笼 5只,人工光照时间为明暗交替12 h。

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