响应面法优化复合酶提取枸杞子总黄酮的工艺研究

高建德,朱晓玉,刘雄,陈晖,宋开蓉,师婷婷

CJI (Traditional Chinese Medicine) - - Bcl-2 -

甘肃中医药大学药学院,甘肃 兰州 730000

摘要:目的 采用响应面法优化复合酶提取枸杞子总黄酮的工艺条件。方法 在单因素试验基础上,选择酶用量、酶解时间、酶解温度为因素,进行3因素 3水平的中心组合设计,采用响应面法分析3个因素对枸杞子总黄酮得率的影响。结果 复合酶辅助提取枸杞子总黄酮的工艺路线为:复合酶用量0.30%,酶解时间 1.0 h,酶解温度 60 ℃。总黄酮得率预测值为 0.949 2%。结论 响应面法优化复合酶辅助提取枸杞子总黄酮的方法稳定可行,操作简单,可为深入研究及应用提供依据。关键词:响应面法;工艺优化;复合酶提取;枸杞子总黄酮

DOI:10.3969/j.issn.1005-5304.2017.07.016

中图分类号:R284.2 文献标识码:A 文章编号:1005-5304(2017)07-0066-06

Optimization of Compound Enzyme Extraction Technology of Total Flavonoids from Lycii

Fructus by Response Surface Method GAO Jian-de, ZHU Xiao-yu, LIU Xiong, CHEN Hui, SONG Kai-rong, SHI Ting-ting (College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China)

Abstract: Objective To optimize the extraction technology of total flavonoids from Lycii Fructus by response surface methodology (RSM). Methods On the basis of single-factor tests, enzyme concentration, enzymolysis time, and enzyme solution temperature were selected as influencing factors during extraction to conduct three-factor three-level center combination design. The effects of three factors on the yield of total flavonoids from Lycii Fructus were analyzed by RSM. Results The optimum conditions for extraction technology of total flavonoids from Lycii Fructus were enzyme concentration 0.30%, enzymolysis time 1.0 h, enzyme solution temperature 60 ℃. The predicted extraction yield of total flavonoids was 0.949 2%. Conclusion Using RSM to optimize the extraction technology of total flavonoids from Lycii Fructus is stable, feasible, and simple, which can provide references for further study and application.

Key words: response surface method; technology optimization; compound enzyme extraction; total flavonoids from Lycii Fructus

枸杞子是茄科植物宁夏枸杞 Lycium barbarum L.

的干燥成熟果实[1],性平,味甘、微苦,无毒,具有滋养肝肾、润肺益气、生精助阳、明目等功效。黄酮

类化合物是枸杞子的有效组分之一,具有抗肿瘤[2]、

抗氧化[3]、抗衰老[4]、清除自由基[5]、抗炎[6]等作用。目前,黄酮类化合物的提取方法主要有回流提取

法[7-8]、超声提取法[9-10]、微波辅助提取法[11]及 CO2

超临界流体萃取法[12]。生物酶技术具有提取效率高、

提取时间短、绿色环保及提取条件温和等优势[13],近年来在中药黄酮类、多糖类、生物碱类等有效组分提取中均有广泛应用。生物酶辅助提取枸杞子的有效组

分虽有报道,但以研究提取枸杞子多糖[14-15]为主,而在提取枸杞子黄酮类成分方面报道甚少。本研究在单因素试验的基础上,采用响应面法优化复合酶提取枸杞子总黄酮的工艺,为枸杞子的综合利用及提高黄酮类化合物的提取率提供一定的试验基础。

1 仪器与试药

UV Blue Star B型紫外-可见分光光度计(北京莱佰泰科技有限公司),DD-5M离心机(湘仪离心机仪器有限公司), AL104 型电子天平( METTLER

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