李道睿1，王苗苗 1，2，于明薇 3，林飞 1，张颖 1，李蒙 1，樊慧婷 1，郑琦 1，祁鑫1，裴迎霞 1，张培彤 1，侯炜 1，林洪生 1
1.中国中医科学院广安门医院，北京 100053；2.北京中医药大学，北京 100029；
摘要：目的 观察通络活血虫类药（全蝎、蜈蚣、守宫）对乏氧环境下肺癌血管生成相关细胞因子的影响。方法 采用低氧工作站体外模拟肿瘤乏氧微环境培养A549肺癌细胞，加入不同浓度全蝎、蜈蚣、守宫药物血清，MTT法检测细胞增殖，筛选最佳药物浓度及作用时间。A549肺癌细胞经3种药物血清处理后，收集细胞培养上清液，ELISA 检测上清液中血管内皮生长因子（VEGF）、转化生长因子-β1（TGF-β1）、碱性成纤维细胞生长因子（bFGF）的含量。结果 3种药物血清对常氧及乏氧培养的A549肺癌细胞增殖均有一定的抑制作用。最终筛选7.5%浓度药物血清、作用 24 h进行后续实验。3种药物血清较对照组A549 细胞 VEGF、TGF-β1、bFGF 含量均降低（P＜0.05，P＜0.01）。结论 3种虫类药在常氧和乏氧状态下均有抑制癌细胞和对血管生成相关细胞因子调控的作用。
中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)09-0039-04
Effects of Dredging Collaterals and Activating Blood Worm Chinese Materia Medica on Angiogenesis Related Factors of Lung Cancer in Hypoxic Environment
LI Dao-rui1, WANG Miao-miao1,2, YU Ming-wei3, LIN Fei1, ZHANG Ying1, LI Meng1, FAN Hui-ting1, ZHENG Qi1, QI Xin1, PEI Ying-xia1, ZHANG Pei-tong1, HOU Wei1, LIN Hong-sheng1 (1. Guang’anmen Hospital, China Academy of Chinses Medical Sciences, Beijing 100053, China; 2. Beijing University of Chinese Medicine, Beijing 100029, China; 3. Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China)
Abstract: Objective To observe the effects of dredging collaterals and activating blood worm Chinese materia medica on angiogenesis related factors of lung cancer in hypoxic environment. Methods The lung cancer A549 cells were cultured in vitro to simulate tumor hypoxia microenvironment by the hypoxia workstation, and different concentrations of Scorpio, Scolopendra and Gecko medicated serum were added. MTT method was used to detect cell proliferation and screen the best medicine concentration and duration of action. Lung cancer A549 cells were administrated by the three kinds of medicated serum, and cells were collected and supernatant was cultured. Contents of VEGF, TGF-β1, and bFGF were detected by ELISA. Results Three kinds of medicated serum had the inhibitory effect on both added normoxia and hypoxia in cultured A549 lung cancer cells. 7.5% concentration of medicated serum was selected, and 24 h later were used in later experiments. Scorpio, Scolopendra and Gecko medicated serum can more reduce the contents of VEGF, TGF-β1 and bFGF in the supernatant of A549 cell compared with the control group (P<0.05, P<0.01). Conclusion Dredging collaterals and activating blood worm Chinese materia medica had inhibitory effect on cancer cells and the regulation of angiogenesis related cytokines in the condition of normoxia and hypoxia.
Key words: dredging collaterals and activating blood; worm Chinese materia medica; lung cancer; hypoxia