柳茶总三萜提取富集工艺优化及抗缺氧活性研究

CJI (Traditional Chinese Medicine) - - 中国中医药信息杂志 -

王昕1,葸慧荣2,杨芳芳2,何蕾2,马慧萍 1

1.甘肃中医药大学药学院,甘肃 兰州 730000;

2.兰州军区兰州总医院全军高原损伤防治重点实验室,甘肃 兰州 730050

摘要:目的 优选藏药柳茶总三萜的提取富集工艺并研究其抗缺氧活性。方法 通过小鼠常压密闭缺氧实验筛选柳茶总三萜的提取溶剂;再以总三萜含量、提取率为评价指标,采用正交试验考察提取时间、提取次数、料液比,优选柳茶总三萜的提取工艺;然后考察大孔吸附树脂类型、上样浓度、洗脱剂浓度、洗脱剂体积,优选其富集工艺;最后通过小鼠常压密闭缺氧实验和急性减压缺氧实验研究其抗缺氧活性。结果 优选的提取工

艺为:70%乙醇提取,料液比为 1∶20,提取 2 次,每次 1.5 h,平均总三萜含量可达 32.25%;优选的富集工

艺为:AB-8型大孔吸附树脂,上样浓度为2 mg/mL,80%乙醇 5 BV洗脱,总三萜含量可达61.09%;柳茶总三萜抗缺氧活性较强,且存在一定的剂量依赖性。结论 本研究优选的柳茶总三萜提取富集工艺稳定、重现性好,所得柳茶总三萜具有显著的抗缺氧活性。关键词:柳茶;总三萜;提取工艺;富集工艺;抗缺氧

DOI:10.3969/j.issn.1005-5304.2018.04.015

中图分类号:R284.2;R285.5 文献标识码:A 文章编号:1005-5304(2018)04-0071-06

Optimization of Extraction and Enrichment Process and Anti-hypoxia Activity of Total Triterpenes from Sibiraea Angustata

WANG Xin1, XI Hui-rong2,YANG Fang-fang2, HE Lei2, MA Hui-ping1

1. Pharmacy College of Gansu University of Chinese Medicine, Lanzhou 730000, China;

2. Key Laboratory of Prevention and Cure for the Plateau Environment Damage of PLA, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, China

Abstract: Objective To optimize the extraction and enrichment process of total triterpenes from Sibiraea Angustata; To study its anti-hypoxia activity. Methods The extraction solvent of total triterpenoids of Sibiraea Angustata was screened by atmospheric pressure anti-hypoxia test. The content of total triterpenes and extraction rate were set as evaluation indexes. The effects of extraction time, extraction times and ratio of material to liquid on extraction were investigated by orthogonal test. The extraction process was optimized. Resin type, concentration of sample, eluent concentration and eluent volume were investigated to optimize the enrichment process of total triterpenes of Sibiraea Angustata. Finally, the anti-hypoxia activity was studied by atmospheric pressure test and acute decompression test. Results The optimum extraction process was as follows: 70% ethanol extraction, the ratio of material to liquid was 1:20, extrat 2 times with 1.5 h for each time, and the content of total triterpenes was 32.25%. The optimum enrichment process was: AB-8-type macroporous adsorption resin, the loading concentration of 2 mg/mL,

80% ethanol 5 BV elution, and total triterpenes content was up to 61.09%; Sibiraea Angustata total triterpenes anti-hypoxia activity test was strong, and had a certain dose dependency. Conclusion The extraction and enrichment process of total triterpenes from Sibiraea Angustata is stable, reproducible and has significant anti-hypoxia activity.

Keywords: Sibiraea Angustata; total triterpenes; extraction process; enrichment process; anti-hypoxia

柳茶是蔷薇科Rosaceae 鲜卑花属 Sibiraea Maxim植物窄叶鲜卑花 Sibiraea angustata ( Rehd. ) Hand-Mazz.的叶和嫩枝,是我国藏族民间传统药材,生长在海拔 3000~5000 m的高原地带,分布在青海、西藏、云南及甘肃等地区,资源丰富。民间将窄叶鲜

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