镇癫开窍颗粒定性定量方法研究

CJI (Traditional Chinese Medicine) - - 中国中医药信息杂志 -

张瑞堂 1,2,金永新 2,张红梅2,马肖2,何彩虹2,田新华2,吴玉琼 1,2

1.西北民族大学,甘肃 兰州 730000;2.甘肃省第二人民医院,甘肃 兰州 730000

摘要:目的 建立镇癫开窍颗粒的定性定量方法。方法 采用薄层色谱法鉴别制剂中的龙胆、黄芩、大黄、白芍。采用 HPLC对制剂中的龙胆苦苷、芍药苷进行含量测定,色谱柱为 Phenomenex C18 柱(250 mm×4.6 mm,

5 μm),流动相为甲醇-水(12∶88),流速为 1.0 mL/min,检测波长为 273 nm(龙胆苦苷)、230 nm(芍药苷),柱温为室温。结果 薄层色谱能检出龙胆、黄芩、大黄、白芍。龙胆苦苷在2.396~11.980 —g范围内呈良好的

线性关系(r=0.999 6),平均加样回收率为 99.72%(RSD=2.70%);芍药苷在 2.728~13.640 —g范围内呈良

好的线性关系(r=0.999 0),平均加样回收率为 98.74%(RSD=2.42%)。结论 本研究所建立的方法简便、可靠、重复性好,可用于控制镇癫开窍颗粒的质量。关键词:镇癫开窍颗粒;龙胆苦苷;芍药苷;龙胆;薄层色谱法;高效液相色谱法

DOI:10.3969/j.issn.1005-5304.2018.04.016

中图分类号:R284.1 文献标识码:A 文章编号:1005-5304(2018)04-0077-05

Study on Qualitative and Quantitative Analysis of Zhendian Kaiqiao

ZHANG Rui-tang1,2, JING Yong-xin2, ZHANG Hong-mei2, MA Xiao2,

HE Cai-hong2, TIAN Xin-hua2, WU Yu-qiong1,2

1. Northwest University for Nationalities, Lanzhou 730000, China;

2. Second People’s Hospital of Gansu Province, Lanzhou 730000, China

Abstract: Objective To establish a method for qualitative and quantitative analysis of Zhendian Kaiqiao Granules. Methods Gentiane Radix et Rhizoma, Scuteliariae Radix, Rhei Radix et Rhizoma, Radices Paeoniae Alba in the preparation were identified by TLC. Gentiopicrin and paeoniflorin were determined by HPLC. The analysis was performed on a Phenomenex C18 column (250 mm × 4.6 mm, 5 μm), with the mobile phase of methyl alcohol-water

(12:88). The flow rate was 1.0 mL/min and the column temperature was maintained at room temperature; the detection wavelengths were set at 273 nm (gentiopicrin) and 230 nm (paeoniflorin). Results Gentiane Radix et Rhizoma, Scuteliariae Radix, Rhei Radix et Rhizoma, Radices Paeoniae Alba could be detected by TLC. Gentiopicrin showed a good linear relationship at a range of 2.396–11.980 —g, r=0.999 6. The average recovery was 99.72%, and RSD was 2.70%. Paeoniflorin showed a good linear relationship at a range of 2.728–13.640 —g, r=0.999 0. The average recovery was 98.74%, and RSD was 2.42%. Conclusion The established method is simple, reliable and reproductive. The method can be used for control quality of Zhendian Kaiqiao Granules.

Keywords: Zhendian Kaiqiao Granules; gentiopicrin; paeoniflorin; Gentiane identification; HPLC

镇癫开窍颗粒处方来源于甘肃省第二人民医院(甘肃省精神卫生中心)中医科,为该院经验方,经多年临床使用效果明确、毒副作用小。为使患者携带方便,拟开发为医院院内制剂。该制剂由龙胆、黄芩、白芍、大黄、石膏、生地黄、酸枣仁、龙骨、牡蛎、珍珠母、石决明、琥珀、细辛、茯苓、甘草共15 味

基金项目:中央高校基本科研业务费专项(31920150095)

通讯作者:吴玉琼,E-mail:wyq-997@163.com Radix et Rhizoma; TLC 药组成,具有泄肝潜镇、清泄阳明功效,用于治疗癫狂症。为了控制镇癫开窍颗粒的质量,本研究采用薄层色谱方法对制剂中的龙胆、白芍、黄芩、大黄进行定性鉴别,采用HPLC对制剂中的龙胆苦苷、芍药苷进行含量测定,为将镇癫开窍颗粒开发成安全、有效、价廉的医院院内制剂提供依据。仪器与试药型高效液相色谱仪(二极管阵列检测

三用紫外分析仪(上

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