Ja­panese sci­en­tists de­velop high res­o­lu­tion imag­ing tech­nique

BioSpectrum (Asia) - - Science News -

Osaka Univer­sity led re­searchers have de­vel­oped block­face serial mi­croscopy to­mog­ra­phy (FAST), an imag­ing sys­tem that can image a whole mouse brain at high spa­tial res­o­lu­tion in less than two-and-a-half hours. FAST con­sists of a spin­ning disk con­fo­cal mi­cro­scope with built in mi­croslicer and a method for pro­cess­ing image data. With the 3D re­con­struc­tion tech­nique, whole brains can be vi­su­al­ized at a res­o­lu­tion high enough to re­solve in­di­vid­ual cells and their sub­cel­lu­lar struc­tures. By com­bin­ing their FAST tech­nique with spe­cific stain­ing pro­ce­dures, the team of sci­en­tists were able to vi­su­al­ize sub­cel­lu­lar nu­clei, vas­cu­lar struc­tures, ma­ture oligo­den­dro­cytes, myelin sheaths, in­terneu­rons, and pro­ject­ing neu­rons through­out the whole brain. These imag­ing tools pro­vide a sys­temic ap­proach to in­ves­ti­gat­ing the patho­phys­i­o­log­i­cal mech­a­nisms of dif­fer­ent brain dis­eases. This new tech­nique of­fers a way to com­pare mul­ti­ple brains at the level of in­di­vid­ual cells and their sub­cel­lu­lar struc­tures. With this ap­proach, new in­sights will be gained into the patho­log­i­cal mech­a­nisms of dif­fer­ent brain dis­eases. Fur­ther­more, the ap­pli­ca­bil­ity of this of­fers a trans­la­tional ap­proach to re­search­ing non­hu­man an­i­mal mod­els and hu­man dis­eases.

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