Thermo Fisher con­tin­ues to ex­pand its DNA iso­la­tion kits

BioSpectrum (Asia) - - Supplier News -

Thermo Fisher Sci­en­tific Inc., the world leader in serv­ing science, an­nounced the launch of its new PureLink Fast Low-En­do­toxin Plas­mid Pu­rifi­ca­tion Midi and Maxi Kits, fur­ther strength­en­ing its line of low-en­do­toxin plas­mid DNA iso­la­tion prod­ucts. The kits of­fer re­searchers dra­mat­i­cally ac­cel­er­ated iso­la­tion of plas­mid DNA com­pared to cur­rent stan­dard pro­to­cols. With the PureLink kits, re­searchers can iso­late trans­fec­tion-qual­ity plas­mid in about 30 min­utes, up to 0.4 mg for midi scale and up to 1.5 mg for maxi scale prepa­ra­tion, en­abling re­searchers to ob­tain high yields of low-en­do­toxin DNA in sig­nif­i­cantly less time and with­out a te­dious al­co­hol pre­cip­i­ta­tion step. The tech­nol­ogy be­hind the faster iso­la­tion time is Thermo Fisher’s in­no­va­tive ad­vanced sil­ica mem­brane-based col­umns, uti­lized in com­bi­na­tion with the Thermo Sci­en­tific FastVac vac­uum man­i­fold, en­abling iso­la­tion of trans­fec­tion-qual­ity plas­mid in as lit­tle as 30 min­utes. Tra­di­tional an­ion-ex­change resin-based col­umns rely on grav­ity flow to pu­rify the DNA, and also re­quire an iso­propanol pre­cip­i­ta­tion step, which takes more than two hours.

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