Proving the action of the combined product
A 25-day trial was conducted to study the effects of dietary supplementation with a blend of formic, propionic and acetic acids combined with cinnamaldehyde and a permeabilizing complex (BTR, Biotronic® Top3, BIOMIN, Austria) on the reduction of
S. Enteritidis counts in White Leghorn cecum. From day of hatch, 60 day-old White Leghorn SPF chickens were randomly assigned to 3 treatments of 20 chicks each and placed in poultry isolators. A control group received a commercial broiler diet with no antibiotic or BTR, trial group 1 (BTR 1) received 1 kg Btr/ton of feed, and trial group 2 (BTR 2) received 2 kg BTR/ ton. At day 15, chickens were eye-drop inoculated with 105 colony-forming units (CFU) of S. Enteritidis field strain 208876/201. At day 5 (20 days of age) and 10 (25 days of age) post infection (dpi), 10 chickens per group were euthanized and cecum content was taken for bacteriological analyses (Table 1). The CFU per gram of cecum sample was determined by counting the bacterial colonies. The results of the study showed a significant (P<0.05) reduction in S. Enteritidis counts in the cecal content of birds.