Analysis on Microbial Diversity in the Sediments and Its Relationship with Environmental Factors in a Reservoir

ZHU Tingting1, TIAN Congkui2,†

1. State Environmental Protection Key Laboratory of Drinking Water Source Management and Technology, Shenzhen Key Laboratory of Drinking Water Source Safety Control, Shenzhen Key Laboratory of Emerging Contaminants Detection and Control in Water Environment, Shenzhen Academy of Environmental Sciences, Shenzhen 518001; 2. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Hubei University for Nationalities, Enshi 445000; † Corresponding author, E-mail: pkutianck@163.com

Abstract In order to reveal the correlation between microbial diversity and the pollution degrees of the source water in a reservoir in Guangdong Province, for better understanding the ecological functions of this region, the environmental factors and microbial community were analyzed based on the characterization of the data from six sampling sites. The microbial diversity of different points was analysed, using PCR amplification technology and T-RFLP technology. Generally, the physical and chemical properties of each sampling point in the interstitial water in the reservoir showed obvious difference. Different forms of nitrogen concentrations and organic carbon concentrations in the reservoir were lower than those of the external diversion and the tributary. The concentrations of the enviromental factors were in the orders of wet season>dry season>normal season. The result showed that bacterial community structures varied considerably between the upstream and the downstream. The source water