按经选穴针刺对失眠大鼠下丘脑-垂体-肾上腺皮质轴相关激素的影响

吴雪芬，岳增辉，郑雪娜，郭鑫，谢志强，谢莉娜

湖南中医药大学，湖南 长沙 410007

摘要：目的 观察按经选穴针刺对失眠大鼠下丘脑-垂体-肾上腺皮质（HPA）轴相关激素含量及腧穴配伍效应的影响，探讨针刺治疗失眠症的作用机制。方法 腹腔注射对氯苯丙氨酸混悬液建立失眠模型大鼠。将

60 只 SD大鼠随机分为空白组、模型组、百会＋神门组、百会＋三阴交组、百会＋非经非穴组，每组12 只。各治疗组针刺相应腧穴，每次30 min，连续 7 d。ELISA检测下丘脑促肾上腺皮质激素释放激素（CRH）及血清促肾上腺皮质激素（ACTH）、皮质酮（CORT）的含量。结果 与模型组比较，各针刺组大鼠下丘脑 CRH及血清 ACTH、CORT的含量均有一定程度降低；各针刺组间比较，百会＋神门组的疗效优于百会＋三阴交组及百会＋非经非穴组。结论 针刺可能通过调节 HPA 轴相关激素水平起到镇静安神的作用，按经选穴针刺可能是影响腧穴配伍效应的影响因素之一。关键词：针刺；失眠；下丘脑-垂体-肾上腺皮质轴；按经选穴；腧穴配伍；大鼠

DOI：10.3969/j.issn.1005-5304.2017.11.013

中图分类号：R245 文献标识码：A 文章编号：1005-5304(2017)11-0053-05

Effects of Acupuncture Treatment on HPA Axis Related Hormones in Insomnia Rats

WU Xue-fen, YUE Zeng-hui, ZHENG Xue-na, GUO Xin, XIE Zhi-qiang, XIE Li-na (Hunan University of Chinese Medicine, Changsha 410007, China)

Abstract: Objective To observe the effects of acupuncture treatment on the levels of HPA axis related hormones and acupoint compatibility in insomnia rats; To discuss the mechanism of action for acupuncture treatment. Methods Chlorophenylalanine suspension was under intraperitoneal injection to establish insomnia model rats. Sixty SD rats were randomly divided into blank group, model group, Baihui+Shenmen group, Baihui+Sanyinjiao group, Baihui+non-acupoint group, with 12 rats in each group. Each treatment group received acupuncture in relevant acupoints, 30 min each time, for 7 d. ELISA was used to measure the levels of CRH, ACTH and CORT. Results Compared with the model group, the levels of CRH, ACTH and CORT of the acupuncture groups decreased to some extent. In the three acupuncture groups, the efficacy of Baihui+Shenmen group was better than that of Baihui+Sanyinjiao group and Baihui+non-non-acupoint group. Conclusion Acupuncture treatment may calm and soothe the nerves to release the insomnia through regulating HPA axis related hormones. Acupuncture acupoints at different meridians may be one of the factors that cause the difference of acupoints compatibility effect.

Key words: acupuncture; insomnia; HPA axis; acupoints at different meridians; acupoints compatibility; rats

失眠可导致人或动物思维紊乱，常伴有疲劳及不良的情绪变化等症状。自 1969 年国外学者提出 5-羟色胺（5-HT）致眠学说后，有研究发现，下丘脑-垂体-肾上腺皮质（HPA）轴的兴奋-抑制状态是反应睡眠质量的重要指标，正常情况下，睡眠早期非快眼动

基金项目：国家重点基础研究发展计划（2014CB543102）；

国家自然科学基金（81673886）

通讯作者：岳增辉，E-mail：624755064@qq.com 睡眠（NREM）占优势，此时 HPA轴分泌活动受抑制，而睡眠后期快动眼睡眠（REM）占优势，HPA 轴分

泌活动则活跃[1]。氯苯基丙氨酸（PCPA）失眠模型大鼠在下丘脑5-HT含量降低的同时伴有HPA轴的功能紊乱，分布于下丘脑的室旁核和蓝斑核的促肾上腺皮质激素释放因子（CRF），以及血浆促肾上腺皮质激素（ACTH）是直接反映 HPA 轴兴奋-抑制状态的相

关激素[2]，CRF 既可以调控蓝斑核NE系统与背缝核

5-HT系统活性，又作用于下丘脑的CRF受体，调控