样品处理方法对皖南葛根和粉葛中总黄酮和葛根素含量的影响

常跃兴，郭爱灵，陈铎葆，邓云，张荣波，姚斯琪

安徽理工大学医学院药学系，安徽 淮南 232001

摘要：目的 建立葛根和粉葛中总黄酮和葛根素含量测定方法，比较室温超声法、超声加热法和加热回流法等样品处理方法对葛根和粉葛中总黄酮和葛根素含量测定结果的影响。方法 总黄酮含量测定采用紫外分光光度法，在波长250 nm处测定吸光度；葛根素含量测定采用HPLC，固定相为十八烷基硅烷键合硅胶，流动

相为甲醇-水（25∶75），检测波长为 250 nm，流速为 1.0 mL/min。结果 室温超声法、加热回流法和超声加热法葛根中总黄酮含量分别为 15.09%、14.48%、12.71%（n＝3），葛根素含量分别为 4.37%、4.09%、3.80%

（n＝3）；粉葛中总黄酮含量分别为 2.09%、2.23%、2.17%（n＝3），葛根素含量分别为 0.50%、0.53%、0.52%

（n＝3）。结论 室温超声法、超声加热法可替代加热回流法分别用于葛根和粉葛中总黄酮和葛根素含量测定时供试液制备；皖南野生葛根和粉葛中葛根素含量均符合药典标准。关键词：供试液制备；葛根；粉葛；总黄酮；葛根素；含量测定

DOI：10.3969/j.issn.1005-5304.2017.11.019

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)11-0078-04

Effects of Different Sample Preparation Methods on Total Flavonoids and Puerarin Content from Puerariae Lobatae Radix and Puerariae Thomsonii Radix in South Anhui Province

CHANG Yue-xing, GUO Ai-ling, CHEN Duo-bao, DENG Yun, ZHANG Rong-bo, YAO Si-qi (Pharmacy Department of Medical College, Anhui University of Science and Technology, Huainan 232001, China)

Abstract: Objective To establish UV spectrophotometry and HPLC methods for content determinations of total flavonoids and puerarin from Puerariae Lobatae Radix and Puerariae Thomsonii Radix; To compare the ultrasonic method at room temperature, conventional refluxing method and ultrasonic method at heating conditions at the aspect of content determinations. Methods The content determinations of total flavonoids was determined by UV spectrophotometry at 250 nm; the content of puerarin was determined by HPLC with octadecylsilane-bonded silica gel as the stationary phase, a mixture of methanol and water (25:75) as the mobile phase, 256 nm as the detection wavelength, 1.0 mL/min as the flow rate. Results Contents of total flavonoids in Puerariae Lobatae Radix by ultrasonic method at room temperature, conventional refluxing method and ultrasonic method were15.09%, 14.48%, and 12.71% (n=3), respectively. The contents of puerarin were 4.37%, 4.09%, and 3.80% (n=3), respectively. Contents of total flavonoids in Puerariae Thomsonii Radix were 2.09%, 2.23%, and 2.17% (n=3), respectively. The contents of puerarin were 0.50%, 0.53%, and 0.52% (n=3), respectively. Conclusion Ultrasonic method at room temperature can replace conventional refluxing method for content determinations of total flavonoids and puerarin from Puerariae Lobatae Radix, and ultrasonic method at heating conditions also can replace conventional refluxing method for content determinations of total flavonoids puerarin from Puerariae Thomsonii Radix. Puerarin contents from Puerariae Lobatae Radix and Puerariae Thomsonii Radix in South Anhui Province are all in line with the Pharmacopoeia standards.

Key words: sample preparation; Puerariae Lobatae Radix; Puerariae Thomsonii Radix; puerarin; content determination total flavonoids; 基金项目：2015年安徽理工大学引进人才基金项目（11079）；安徽理工大学大学生创新创业训练计划项目（201610361317）