针刺对失眠大鼠血清去甲肾上腺素、多巴胺及5-羟色胺含量的影响

CJI (Traditional Chinese Medicine) - - 中国中医药信息杂志 -

郭鑫,岳增辉,谢菊英,吴雪芬,郑雪娜

湖南中医药大学,湖南 长沙 410208

摘要:目的 观察循经选穴针刺对失眠大鼠外周血清单胺类神经递质5-羟色胺(5-HT)、多巴胺(DA)、去甲肾上腺素(NE)含量及腧穴配伍效应的影响,探讨针刺治疗失眠症的作用机制。方法 将 60 只 SD 大鼠随机分为空白组、模型组、百会+神门组、百会+三阴交组和百会+非经非穴组,每组12只,腹腔注射对氯苯丙氨酸混悬液制作大鼠失眠模型,各针刺组针刺相应腧穴,每次 30 min,连续 7d。ELISA 检测大鼠血清

NE、DA、5-HT 的含量。结果 与空白组比较,模型组大鼠血清5-HT、DA、NE 含量明显升高(P<0.01);与模型组比较,各针刺组大鼠血清DA、NE 含量明显降低(P<0.05,P<0.01),百会+三阴交组大鼠血清 5-HT

含量明显降低(P<0.05);各针刺组间比较,百会+神门组总体疗效优于百会+三阴交组及百会+非经非穴组。结论 针刺改善大鼠睡眠结构可能与血清单胺类神经递质含量有关,循经选穴针刺可能是影响腧穴配伍效应的因素之一。

关键词:针刺;失眠;循经选穴;去甲肾上腺素;多巴胺;5-羟色胺;大鼠

DOI:10.3969/j.issn.1005-5304.2018.04.010

中图分类号:R245 文献标识码:A 文章编号:1005-5304(2018)04-0046-05

Effects of Acupuncture on 5-HT, NE and DA in Serum of Insomnia Rats

GUO Xin, YUE Zeng-hui, XIE Ju-ying, WU Xue-fen, ZHENG Xue-na

Hunan University of Chinese Medicine, Changsha 410208, China

Abstract: Objective To observe the effects of acupuncture in acupoints at different meridians on 5-HT, DA, NE in the peripheral serum of insomnia rats; To explore the mechanism of action of acupuncture for the treatment of insomnia. Methods Sixty SD rats were randomly divided into blank group, model group, Baihui+Shenmen group,

Baihui+Sanyinjiao group, and Baihui+non-acupoint group, with 12 rats in each group. Injection of PCPA suspension of rats was used to establish insomnia model. Each acupuncture group received corresponding acupoints, 30 min each time, for 7 days. The levels of 5-HT, DA and NE in serum were measured by ELISA. Results Compared with the blank group, the levels of 5-HT, DA and NE in the model group were significantly higher than those in the blank group (P<0.01). Compared with the model group, the contents of DA and NE in the acupuncture groups were significantly lower than those in the model group (P<0.05, P<0.01), and the 5-HT in Baihui+Sanyinjiao group was significantly lower than those in the model group (P<0.05). Among all acupuncture groups, overall efficacy of

Baihui+Shenmen group was better than Baihui+Sanyinjiao group and Baihui+non-acupoint group. Conclusion Acupuncture can improve sleep structure in rats may be related to serum monoamine neurotransmitters. Acupuncture in acupoints at different meridians may be one of the influencing factors of acupoint compatibility effects.

Keywords: acupuncture; insomnia; acupoints at different meridians; norepinephrine; dopamine; 5-hydroxy tryptamine; rats 失眠是指入睡困难、睡眠维持障碍和早醒,且对

基金项目:国家重点基础研究发展计划(2014CB543102);

国家自然科学基金(81673886)

通讯作者:岳增辉,E-mail:624755064@qq.com 睡眠时间或质量不满意,继而影响日常功能的一种主

观体验[1]。现代生物学研究认为,自主昼夜节律和睡

眠内稳态驱动共同参与调节睡眠-觉醒周期[2],褪黑素(melatonin,MT)作为一种内源性生理物质,是该节律调节系统的重要组成部分,其与受体结合后,促

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