针刺对自发性高血压大鼠下丘脑室旁核炎症因子含量及 Toll 样受体4表达的影响

CJI (Traditional Chinese Medicine) - - 中国中医药信息杂志 -

姬彩硕1,马思明1,杨娜娜2,田中雪2,杨静雯1,邵佳凯1,王雪蕊1,刘存志 2

1.首都医科大学附属北京中医医院针灸中心,北京 100010;

2.北京中医药大学东方医院,北京 100078

摘要:目的 观察针刺对自发性高血压大鼠(SHR)下丘脑室旁核(PVN)炎症因子含量及Toll 样受体4

(TLR4)表达的影响,探讨其可能的作用机制。方法 10 周龄 SHR 30只,随机分为SHR组、针刺组和非穴组,每组 10 只,另取同周龄WKY 大鼠 10只为正常组。针刺组针刺双侧“太冲”,采用捻转泻法刺激,非穴

组针刺其足背侧,采用平补平泻捻转法刺激,2周后取材。每日检测1 次大鼠平均动脉压;RT-PCR检测 PVN中TLR4 mRNA的表达;Western blot检测PVN中TLR4蛋白的表达;ELISA检测PVN肿瘤坏死因子-α(TNF-α)、

白细胞介素-6(IL-6)的含量。结果 与正常组比较,SHR 组大鼠平均动脉压升高,PVN 中 TLR4 mRNA 和

蛋白表达明显升高,TNF-α、IL-6 含量明显增加(P<0.05);与 SHR 组比较,针刺组大鼠平均动脉压明显下降,PVN 中 TLR4 mRNA 和蛋白表达明显降低,TNF-α、IL-6 含量明显减少(P<0.05);而非穴组无明显变化。结论 针刺“太冲”可能通过抑制 SHR PVN 中 TLR4 的表达,减少 TNF-α、IL-6 分泌,从而降低 SHR血压。

关键词:针刺;太冲;高血压;Toll样受体4;炎症因子;自发性高血压大鼠

DOI:10.3969/j.issn.1005-5304.2018.04.013

中图分类号:R245 文献标识码:A

Effects of Acupuncture on the Level of Inflammatory Cytokines and Toll-like Receptor 4 in Hypothalamic Paraventricular Nucleus of Spontaneously Hypertensive Rats

JI Cai-shuo1, MA Si-ming1, YANG Na-na2, TIAN Zhong-xue2, YANG Jing-wen1, SHAO Jia-kai1,

WANG Xue-rui1, LIU Cun-zhi2

1. Beijing Hospital of TCM Affiliated to Capital Medical University, the Acupuncture Center, Beijing 100010, China;

2. Dongfang Hospital, Beijing University of Chinese Medicine, Beijing 100078, China

Abstract: Objective To observe the effects of acupuncture on the level of inflammatory cytokines and toll-like receptor 4 in hypothalamic paraventricular nucleus (PVN) of SHR; To investigate the mechanisms of acupuncture in reduction of mean arterial blood pressure (MAP) in SHR. Methods Thirty 10-week old SHR were randomly divided into SHR group, acupuncture group and non-acupoint group, with 10 rats in each group. 10 WKY rats were set as control group. Acupuncture group received bilateral acupuncture in “Taichong” acupoint, and twisting and diarrhea method was used to stimulate; non-acupoint group received acupuncture at the back of feet, and soothing and diarrhea with twisting method was used to stimulate. Materials were taken two weeks later. The mean arterial pressure of rats was detected every day; the expression of TLR4 mRNA in PVN was detected by RT-PCR; The expression of TLR4 protein in PVN was detected by Western blot; The levels of TNF-αa nd IL-6 were detected. Results Compared with control group, the mean arterial pressure of SHR group increased; TLR4 mRNA and protein expression in PVN increased significantly; the level of TNF-α and IL-6 increased significantly

(P<0.05). Compared with SHR group, the mean arterial pressure of acupuncture group decreased significantly;

TLR4 mRNA and protein expression in PVN decreased significantly; the level of TNF-α and IL-6 decreased

基金项目:北京市自然科学基金(KZ201710025025)

通讯作者:刘存志,E-mail:lcz623780@126.com 文章编号:1005-5304(2018)04-0062-04

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