健脾消癌方对人结肠癌HCT116细胞周期、凋亡及 Wnt/β-catenin 信号通路相关因子的影响

杨晓1，唐蔚2，蒋益兰2，陶子豪1，刘科 1，宋程 3

1.湖南中医药大学研究生院，湖南 长沙 410208；2.湖南省中医药研究院附属医院，湖南 长沙 410006；

3.湖南省肿瘤医院，中南大学湘雅医学院附属肿瘤医院，湖南 长沙 410006

摘要：目的 观察健脾消癌方对 Wnt/β-catenin 信号通路相关因子及人结肠癌 HCT116 细胞周期、凋亡的影响，探讨其抗大肠癌复发转移的作用机制。方法 将对数生长期的 HCT116细胞分为空白组、生理盐水组、

5-Fu 组及健脾消癌方低、中、高剂量组，分别干预 48 h，收集细胞，采用流式细胞术检测细胞周期、凋亡， Western blot 检测细胞核内 β-catenin 蛋白表达，PCR检测 c-myc、cyclinD1 mRNA表达。结果 与空白组及生理盐水组比较，健脾消癌方各剂量组 HCT116 细胞凋亡比例、G1 期细胞比例增加，S 期细胞比例降低，且其细胞核内 β-catenin 蛋白表达降低，c-myc、cyclinD1 mRNA表达降低，尤以健脾消癌方高剂量组明显，差异有

统计学意义（P＜0.05，P＜0.01）。结论 健脾消癌方可促进人结肠癌HCT116细胞凋亡并将其细胞周期阻滞，其机制可能与调节 Wnt/β-catenin 信号通路有关。

关键词：健脾消癌方；大肠癌；Wnt/β-catenin 信号通路；细胞凋亡；细胞周期

DOI：10.3969/j.issn.1005-5304.2018.06.015

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2018)06-0061-05

Effects of Jianpi Xiaoai Prescription on Cell Cycle and Apoptosis of Human Colon Cancer HCT116 Cells and Wnt/β-catenin Signaling Pathway Related Proteins

YANG Xiao1, TANG Wei2, JIANG Yi-lan2, TAO Zi-hao1, LIU Ke1, SONG Cheng3

1. Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China;

2. The Affiliated Hospital of Hunan Academy of Chinese Medicine, Changsha 410006, China;

3. Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410006, China

Abstract: Objective To study the effects of Jianpi Xiaoai Prescription on cell cycle and apoptosis of human colon cancer HCT116 cells and related factors of Wnt/β-catenin signaling pathway; To investigate its mechanisms of anti-metastasis and recurrence of colorectal cancer. Methods The logarithmic growth phase HCT116 cells were divided into blank group, saline group, 5-Fu group, and Jianpi Xiaoai Prescription low-, medium-, and high-dose groups. After intervention for 48 h, the cells were harvested, and the cell cycle and apoptosis were detected by flow cytometry. The protein expression of β-catenin in the nucleus was detected by Western blot, and the expression of c-myc and cyclinD1 mRNA was detected by PCR. Results Compared with the blank group and saline group, the ratio of HCT116 cells apoptosis of Jianpi Xiaoai Prescription low-, medium-, and high-dose groups increased; the proportion of cells in phase G1 increased and the proportion of S cells decreased; the expression of β-catenin protein in the nucleus and the expression of c-myc, cyclinD1 mRNA decreased, especially in the Jianpi Xiaoai Prescription high-dose group, with statistical significance (P<0.05, P<0.01). Conclusion Jianpi Xiaoai Prescription can promote apoptosis of human colon cancer HCT116 cells and block the cell cycle, and its mechanism may be related to regulating Wnt/β-catenin signaling pathway.

Keywords: Jianpi Xiaoai Prescription; colorectal cancer; Wnt/β-catenin signaling pathway; apoptosis; cell cycle