基于网络药理学探讨健胃清肠合剂治疗急性胰腺炎作用机制

1.甘肃中医药大学，甘肃 兰州 730000；2.甘肃省中医院，甘肃 兰州 730000

摘要：目的 基于网络药理学探讨健胃清肠合剂治疗急性胰腺炎（AP）的作用机制。方法 基于TCMSP、HERB数据平台检索，筛选健胃清肠合剂主要活性成分；在GeneCards、OMIM、DrugBank数据库获取AP相关疾病靶点，筛选出健胃清肠合剂活性成分对应靶点与AP的共同靶点；通过STRING数据库构建药物-疾病共同靶点的蛋白相互作用网络；利用DAVID平台进行GO功能和KEGG通路富集分析；采用Cytoscape3.9.1软件构建药物-成分-靶点-通路网络，应用网络拓扑学筛选健胃清肠合剂治疗AP的关键活性成分及核心靶点。结果 获得健胃清肠合剂活性成分37个，包括大黄素、槲皮素、川陈皮素等，得到健胃清肠合剂防治AP靶点123个，核心靶点有PTGS2、HSP90AA1、PTGS1等，KEGG通路富集结果显示，健胃清肠合剂主要作用于IL-17、TNF、PI3K-Akt等多条信号通路。结论 健胃清肠合剂可能通过槲皮素、β-谷甾醇、柚皮素、川陈皮素等活性成分作用于PTGS2、HSP90AA1、PTGS1等靶点，进而调控IL-17、TNF、PI3K-Akt、HIF-1等信号通路，以抑制炎症反应、抗氧化、调节自噬、减少细胞凋亡，多成分、多靶点、多通路治疗AP。

关键词：网络药理学；健胃清肠合剂；急性胰腺炎；作用机制

中图分类号：R289

文献标识码：A 文章编号：2095-5707(2024)02-0052-05

DOI：10.3969/j.issn.2095-5707.202303049 开放科学（资源服务）标识码（OSID）：

Exploration on the Mechanism of Jianwei Qingchang Mixture in Treating Acute Pancreatitis Based on

Network Pharmacology

ZHAO Shiwei1, LI Shengcai2, QI Lihong1

（1. Gansu University of Chinese Medicine, Lanzhou 730000, China; 2. Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730000, China）

Abstract: Objective To investigate the mechanism of Jianwei Qingchang Mixture in the treatment of acute pancreatitis (AP) based on network pharmacology. Methods Based on TCMSP and HERB data platforms, the main active components of Jianwei Qingchang Mixture were screened. AP related disease targets were retrieved from GeneCards, OMIM, and DrugBank databases, and common targets for the active components of Jianwei Qingchang

Mixture and acute pancreatitis were screened out; protein interaction networks for drug-disease common targets was constructed through STRING Database; DAVID platform was used for GO function and KEGG pathway enrichment analysis; Cytoscape 3.9.1 software was used to construct a drug-active component-target-pathway network; network topology was used to screen key active components and core targets of Jianwei Qingchang Mixture in the treatment of AP. Results Totally 37 active components of Jianwei Qingchang Mixture were obtained, including emodin,