Diversity of Yeasts on Cabernet Sauvignon Grape Skin from Minquan, Henan

Liquor-Making Science and Technology - - Contents - ZHANG Junjie

ZHANG Junjie1,2, SHANG Yimin2, CHENG Dawei1, SONG Yuting2, YANG Xu2, CHEN Jinyong1 and LIU Chonghuai1 (1. Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, Henan 450009; 2. School of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou, Henan 450002, China)

Abstract: 68 yeast strains were separated by enrichment separation from Cabernet Sauvignon grape skin from vineyard in Minquan,

5.8S- Henan. The diversity of these strains was studied by using D1/D2 and ITS region sequences of 26S rDNA combined with morphological features. A total of 6 known populations of 4 Saccharomyces spieces were identified including Hanseniaspora opuntiae, Hanseniaspora guilliermondii, Hanseniaspora vineae, Saturnispora diversa, Pichia terricola, and Issatchenkia terricola. Among them, Hanseniaspora opuntiae, Hanseniaspora vineae and Saturnispora diversa were the dominant strains, while the content of Hanseniaspora guilliermondii was the least. The results showed that Cabernet Sauvignon grape skin from Minquan contained varieties of yeasts (especially Saturnispora diversa), and the distribution of different species of yeast in quantity varied greatly. Key words: Cabernet Sauvignon; yeasts; 26S rDNA D1/D2; 5.8S-ITS; diversity

民权县位于河南省东部,地处黄河中下游,以北高滩地,堤南以青沙、沙碱为主。年平均气温黄河故堤为界,南北形成两个不同的地形地貌,堤14.1 ℃,年平均降水量 674 mm,无霜期213 d。赤

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