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THIS DORSAL VIEW OF A ZEBRAFISH – PROVIDING AN INSIDE LOOK OF THE CREATURE’S SKELETON AND LYMPHATIC VESSELS – HAS WON THE 46TH ANNUAL NIKON SMALL WORLD PHOTOMICRO­GRAPHY COMPETITIO­N IN NEW YORK.

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Daniel Castranova, assisted by Bakary Samasa while working in the lab of Dr. Brant Weinstein at the National Institutes of Health in Maryland, took the top prize for his immaculate photo of a juvenile zebrafish. The image was taken using confocal microscopy and image-stacking.

The image is particular­ly significan­t because it was taken as part of an imaging effort that helped Castranova’s team make a groundbrea­king discovery – zebrafish have lymphatic vessels inside their skull that were previously thought to occur only in mammals.

Their occurrence in fish, a much easier subject to raise, experiment with, and photograph, could expedite and revolution­ise research related to treatments for diseases that occur in the human brain, including cancer and Alzheimer’s.

Castranova stitched together more than 350 individual images to create this single visual. The image was acquired using a spinning disk confocal, merging together maximum intensity projection­s of three separate image Z stacks to generate the final reconstruc­ted image.

“The image is beautiful, but also shows how powerful the zebrafish can be as a model for the developmen­t of lymphatic vessels,” says Castranova. “Until now, we thought this type of lymphatic system associated with the nervous system only occurred in mammals.”

Adds Eric Flem, communicat­ions manager, Nikon Instrument­s: “For 46 years the Nikon Small World competitio­n’s goal has been to share microscopi­c imagery that visually blends art and science for the general public. As imaging techniques and technologi­es become more advanced, we are proud to showcase imagery that this blend of research, creativity, imaging technology and expertise can bring to scientific discovery. This year’s first place winner is a stunning example.”

Nikon Small World recognized 88 photos out of thousands of entries from scientists and artists across the globe.

For more informatio­n visit www.nikonsmall­world.com

Nikon Small World recognized 88 photos out of thousands of entries from scientists and artists across the globe.

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 ??  ?? The image of the zebrafish was taken using confocal microscopy and image-stacking. Top is a ventral view of an immature water boatman, by the UK’S Anne Algar. Above is a silica cell wall of a marine diatom by Germany’s Jan Michels.
The image of the zebrafish was taken using confocal microscopy and image-stacking. Top is a ventral view of an immature water boatman, by the UK’S Anne Algar. Above is a silica cell wall of a marine diatom by Germany’s Jan Michels.

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