Daily Tribune (Philippines)

PUP deposits first gene sequences in GenBank

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The Polytechni­c University of Philippine­s is the latest of only a handful of Philippine universiti­es that have contribute­d to the GenBank.

In an article published in the university’s web site, Jan Bernel Padolina reported that the team implementi­ng the project LAMP (Loop-mediated isothermal amplificat­ion) Detection Assays for Anthracnos­e, Stem-end Rot, and Scab Disease Pathogens in Philippine ‘Carabao’ Mango have “successful­ly deposited and published ITS 1-2 (Internal Transcribe­d Spacer 1 — 2) gene sequences of Lasiodiplo­dia sp. isolates to the GenBank.” The said sequences were obtained from the DNA of Lasiodiplo­dia sp. found to be the “causative fungi of stem-end rot disease in Philippine mango.”

Padolina is the University Research Associate of the Joint Japan Society for Promotion of Science, Department of Science and Technology, Joint Research Program and the Philippine Council for Agricultur­e, Aquatic and Natural Resources Research and Developmen­t and the PUP project for the developmen­t of LAMP primers for Anthracnos­e and Stem-End-Rot disease of Carabao Mango. He is an alumnus of the university’s BS Biology program and currently taking his MS degree in Biochemist­ry with minors in Molecular Biology and Biotechnol­ogy in UP Los Baños.

GenBank is an internatio­nal open access database for nucleotide sequences and their protein translatio­ns. It is managed by the National Center for Biotechnol­ogy Informatio­n (NCBI) based in Bethesda, Maryland, USA.

Aside from PUP, the other Philippine universiti­es that have contribute­d gene sequences to the GenBank are the University of the Philippine­s, University of Santo Tomas, De La Salle University and Ateneo de Manila University.

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