Aphyosemion and Fundulopanchax breed quite readily in the aquarium. They don’t need particularly soft or acidic water – for best results a ph of 6.0 to 6.5 is ideal, but instances of them breeding in a ph of 8.0 isn’t unheard of.
Add a pair or a male-female-female trio in something like a 20l tank with spawning mops around it to simulate plant life. The temperature should be 21-24°C and high-protein live and frozen foods fed to condition the brood stock.
Killifish lay small amounts of eggs daily over a week or two and a healthy batch should result in 4060 fry. The eggs can be collected from the spawning mops and either incubated in water or in damp peat/ coconut fibres for transportation – keep them dark in either situation.
Egg fungus can be an issue when eggs are kept in water, but a low dose of methylene blue will help to resolve that. Daily removal of any white eggs is needed along with regular, small water changes.
The developing fry use all the yolk while in their eggs and are ready to eat brineshrimp nauplii straight after hatching. Growth rates are fast and the young can soon be slowly conditioned to harder water.
Even with no intervention it’s still probable that a few odd eggs will develop and produce some fry in a ‘permanent’ set-up. With heavy planting there’s a chance they could survive to maturity but this leaves much to luck.