1915 EXPEDITION DISCOVERED TOMB; MUMMY’S DNA EXTR ACTED IN 2016
old mummy, they would add a powerful DNA collecting technique to their forensics arsenal and also unlock a new way of deciphering Egypt’s ancient past.
“I honestly didn’t expect it to work because at the time there was this belief that it was not possible to get DNA from ancient Egyptian remains,” said Odile Loreille, a forensic scientist at the FBI. But in the journal Genes in March, Loreille and her colleagues reported that they had retrieved ancient DNA from the head. And after more than a century of uncertainty, the mystery of the mummy’s identity had been laid to rest.
What lies in Tomb 10A
Djehutynakht and his wife, Lady Djehutynakht, are believed to have lived around 2000 B.C. during Egypt’s Middle Kingdom. They ruled a province of Upper Egypt. Though the walls in their tomb were bare, the coffins were embellished with beautiful hieroglyphics of the afterlife.
“His coffin is a classic masterpiece of Middle Kingdom art,” said Marleen De Meyer, assistant director for archaeology and Egyptology at the Netherlands-flemish Institute in Cairo, who re-entered the tomb in 2009. “It has elements of a rare kind of realism.”
The team that discovered Djehutynakht’s desecrated chamber more than a century ago was led by the archaeologists George Reisner and Hanford Lyman Story. As they explored the cliffs of Deir el-bersha, which is about 180 miles south of Cairo on the east bank of the Nile, they uncovered a 30-foot burial shaft beneath boulders. With the help of dynamite, they entered the tomb.
In their original reports, the archaeologists said that the dismembered body parts belonged to a woman, presumably Lady Djehutynakht. De Meyer suspected that the head belonged to the governor and not his wife.
Missing Facial Bones
As Freed prepared the items from Tomb 10A for exhibition in 2005, she reached out to Massachusetts General Hospital. Its CT scan revealed the head was missing cheek bones and part of its jaw hinge — features that may have potentially provided insight into the mummy’s sex.
“From the outside, you could not tell that the mummy had been so internally tinkered with,” said Dr. Rajiv Gupta, a neuroradiologist at Massachusetts General. “All the muscles that are involved in chewing and closing the mouth, the attachment sites of those muscles had been taken out.”
They now had another mystery: Why did the mummy have these facial mutilations?
Along with Dr. Paul Chapman, a neurosurgeon at the hospital, Gupta hypothesized that they might be part of an ancient Egyptian mummification practice known as the “Opening of the Mouth Ceremony.” The ritual was performed so the deceased could eat, drink and breathe in the afterlife.
“It’s a very specific cut they made,” said Gupta, referring to the surgical removal of part of the mandible. “There’s a precision to it which is what we were surprised by. Someone was actually doing coronoidectomy 4,000 years ago.”
Some doctors and Egyptologists doubted that ancient Egyptians could perform that complex operation with primitive tools.
To show it was possible, Gupta, Chapman and an oral and maxillofacial surgeon performed the bone removal on two cadavers using a chisel and mallet. They drove the chisel between the lips and gums behind the wisdom teeth, and were able to remove the same bones missing in the mummified skull.
Still, the question of the mummy’s identity lingered.
Tooth raiders
The doctors and museum staff determined their best chance of retrieving DNA would be by extracting the mummy’s molar. “The core of the tooth was where the money was,” Chapman said. Teeth often act as tiny genetic time capsules. Researchers have used them to tell the tales of our prehistoric human cousins called Denisovans, as well as to provide insight into the medical history of long dead people.
“The advantage we had is that we had a hole in the neck because the head had been torn off,” said Chapman.
They snaked a long scope with a camera into the back of the mouth. The first tooth they targeted would not budge, so Dr. Fabio Nunes, who was then a molecular biologist at Massachusetts General, switched to a different molar. Sweating, he clamped down with dental forceps, gave it a few wiggles, then a few twists and “pop” — it was free.
“My main concern was: Don’t drop it, don’t drop it, don’t drop it,” he said. After he successfully maneuvered out from the neck, the room exhaled and gazed upon their prize.
“This looked like an absolutely cavity-free, perfectly preserved tooth,” Freed said. “I thought maybe it was Mrs. Djehutynakht who had died in childbirth. Total speculation.”
FBI tackles an ancient forensic case
For several years, other teams of scientists tried fruitlessly to get DNA from the molar. Then the crown of the tooth went to Loreille at the FBI’S lab in Quantico, Va., in 2016.
Loreille had joined the FBI after 20 years of studying ancient DNA. Previously, she had extracted genetic material from a 130,000-year-old cave bear, and worked on cases to identify unknown Korean War victims, a 2-year-old child who had drowned on the Titanic and two of the Romanov children who were murdered during the Russian Revolution (though she was unable to confirm if one was the famed Anastasia).
In the FBI’S clean lab, Loreille drilled into the tooth’s core and collected a tiny bit of powder. She then dissolved the tooth dust to make a DNA library that allowed her to amplify the amount of DNA she was working with, like a copy machine, and bring it up to detectable levels.
To determine whether what she had extracted was ancient DNA or contamination from modern people, she analyzed how damaged the sample was. It showed signs of heavy damage, confirmation that she was studying the mummy’s genetic material.
She plugged her data into computer software that analyzed the ratio of chromosomes in the sample. “When you have a female you have more reads on X. When you have a male you have X and Y,” she said.
The program spit out “male.” Loreille discovered the mummified severed head had indeed belonged to Djehutynakht. In doing so, she had helped establish that ancient Egyptian DNA could be extracted from mummies.
“It’s one of the Holy Grails of ancient DNA to collect good data from Egyptian mummies,” said Pontus Skoglund, a geneticist at the Francis Crick Institute in London who helped confirm the accuracy of the finding while he was a researcher at Harvard. “It was very exciting to see that Odile got something that looked like it could be authentic ancient DNA.”
Unraveling a mummy’s genetic history
Loreille’s examination also showed that Djehutynakht’s DNA carried clues to another mystery. For centuries, archaeologists and historians have debated the origins of the ancient Egyptians and how closely related they were to modern people living in North Africa. To the researchers’ surprise, the governor’s mitochondrial DNA indicated his ancestry on his mother’s side, or haplogroup, was Eurasian.
“No one will ever believe us,” Loreille recalled telling her colleague Jodi Irwin. “There’s a European haplogroup in an ancient mummy.”
Irwin, the supervisory biologist at the FBI’S DNA support unit, had similar concerns. To verify the results, they sent a portion of the tooth to a Harvard lab, and then to the Department of Homeland Security, for further sequencing.
Then last year as the FBI scientists worked to confirm their results, another group affiliated with the Max Planck Institute for the Science of Human History in Germany reported the first successful extraction of ancient DNA from Egyptian mummies. Their results showed that their ancient Egyptian samples were closer to modern Middle Eastern and European samples than to modern Egyptians, who have more sub-saharan African ancestry.
“It was at the same time ‘Dang! We’re not first,’” Loreille said. “But also we’re happy to see they had this Eurasian ancestry.”
Alexander Peltzer, a population geneticist at the Planck Institute and an author on the first Egyptian mummy DNA paper, said that Loreille’s genetic findings fit well with what his team had found.
“Of course, one has to be careful to deduce too much from single genomes and only two locations,” he said.
Irwin also expressed caution with how the public interprets her team’s results, saying that mitochondrial DNA provides, “just a very small glimpse into somebody’s ancestry.”
Future ancient DNA work will provide insight into how diverse populations moved and mixed in Egypt millenniums ago, according to Verena Schunemann, a paleogeneticist at the University of Zurich in Switzerland who led the Egyptian mummy DNA study that was published before the FBI’S.
Obtaining mummified samples for genetic sequencing may prove difficult for researchers outside of Egypt as the country’s government has barred foreign researchers from taking artifacts and ancient human remains out of the country since 1983. Many investigations will instead rely on museum samples, like Djehutynakht’s decapitated head.
In addition to helping lay groundwork for future exploration of ancient Egypt’s migration history, Loreille and her team’s work may prove beneficial to FBI forensic efforts.
“We are testing techniques that may in the future help them work on remains that are highly degraded, like in the desert or that are burned,” she said.
But for the Egyptologists and medical professionals enthralled by Tomb 10A, the biggest prize was finally solving the mystery of the mummified head.
“You almost feel like it’s a child, like you just identified the gender of a baby,” Nunes said. “It is a boy!”
Freed agreed. “We now know that we have the governor himself,” she said. “We already show the head at the museum, but now we’ll have to change the label!”