Children may carry virus at high levels, study finds
It has been a comforting refrain in the national conversation about reopening schools: Young children are mostly spared by the coronavirus and don’t seem to spread it to others, at least not very often.
But Thursday, a study introduced an unwelcome wrinkle into this smooth narrative.
Infected children have at least as much of the coronavirus in their noses and throats as infected adults, according to the research. Indeed, children younger than age 5 may host up to 100 times as much of the virus in the upper respiratory tract as adults, the authors found.
That measurement does not necessarily prove that children are passing the virus to others. Still, the findings should influence the debate over reopening schools, several experts said.
“The school situation is so complicated. There are many nuances beyond just the scientific one,” said Dr. Taylor Heald-Sargent, a pediatric infectious diseases expert at the Ann and Robert H. Lurie Children’s Hospital of Chicago, who led the study, published in JAMA Pediatrics. “But one takeaway from this is that we can’t assume that just because kids aren’t getting sick, or very sick, that they don’t have the virus.”
The study is not without caveats: It was small and did not specify the participants’ race or sex or whether they had underlying conditions. The tests looked for viral RNA, genetic pieces of the coronavirus, rather than the live virus itself. (Its genetic material is RNA, not DNA.)
Still, experts were alarmed to learn that young children may carry significant amounts of the coronavirus.
“I’ve heard lots of people saying, ‘Well, kids aren’t susceptible; kids don’t get infected.’ And this clearly shows that’s not true,” said Stacey Schultz-Cherry, a virologist at St. Jude Children’s Research Hospital. “I think this is an important, really important, first step in understanding the role that kids are playing in transmission.”
Jason Kindrachuk, a virologist at the University of Manitoba, said, “Now that we’re ... looking at trying to open up schools the next month, this really needs to be considered.”
The standard diagnostic test amplifies the virus’s genetic material in cycles, with the signal growing brighter each round. The more virus present in the swab initially, the fewer cycles needed for a clear result.
Dr. Heald-Sargent, who has a research interest in coronaviruses, began noticing that children’s tests were coming back with low “cycle thresholds,” or CTs, suggesting that their samples were teeming with the virus.
Intrigued, she called the hospital lab on a Sunday and asked to look back at test results for the past several weeks.
“It wasn’t even something we had set out to look for,” she said.
She and her colleagues analyzed samples collected with nasopharyngeal swabs between March 23 and April 27 at drivethrough testing sites in Chicago and from people who came to the hospital for any reason, including symptoms of COVID-19.
They looked at swabs taken from 145 people: 46 children younger than age 5; 51 children ages 5 to 17; and 48 adults ages 18 to 65. To forestall criticisms that really ill children would be expected to have a lot of the virus, the team excluded children who needed oxygen support. Most of the children in the study reported only a fever or cough, Dr. HealdSargent said.
To compare the groups fairly, the team included only children and adults who had mild to moderate symptoms and for whom they had information about when symptoms began. Dr. Heald-Sargent left out people who didn’t have symptoms and who did not remember when they had started to feel ill as well as those who had symptoms for more than a week before the testing.
The results confirmed Dr. Heald-Sargent’s hunch: Older children and adults had similar CTs, with a median of about 11 and ranging up to 17. But children younger than age 5 had significantly lower CTs of about 6.5. The upper limit of the range in these children was a CT of 12, however — still comparable to those of older children and adults.
“It definitely shows that kids do have levels of virus similar to and maybe even higher than adults,” Dr. Heald-Sargent said. “It wouldn’t be surprising if they were able to shed” the virus and spread it to others.
The results are consistent with those from a German study of 47 infected children between the ages of 1 and 11, which showed that children who did not have symptoms had viral loads as high as adults, or higher. And a recent study from France found that asymptomatic children had CT values similar to those of children with symptoms.
CT values are a reasonable proxy for the amount of coronavirus present, said Dr. Kindrachuk, who relied on this metric during the Ebola outbreaks in West Africa.
Still, he and others said that ideally researchers would grow infectious virus from samples, rather than test only for the virus’s RNA.
“I suspect that it probably will translate into meaning that there is more actual virus there as well, but we can’t say that without seeing the data,” said Juliet Morrison, a virologist at the University of California, Riverside.
Some RNA viruses multiply quickly and are prone to genetic errors that render the virus incapable of infecting cells. Some RNA detected in children may represent these “defective” viruses.
“We need to understand how much of that is actually infectious virus,” Dr. Schultz-Cherry said.
(The researchers said they did not have access to the type of high-security lab required to grow infectious coronavirus, but other teams have cultivated the virus from children’s samples.)