Colorado has its first litter of gray wolf pups since the 1940s, state officials said.

Colorado has its first litter of gray wolf pups since the 1940s years, state wildlife officials said Wednesday.

A state biologist and district wildlife manager each spotted the litter of at least three wolf pups over the weekend with their parents, two adult wolves known to live in the state, Gov. Jared Polis announced in a news release. Most wolf litters have four to six pups, so there could be more.

The discovery comes after Colorado voters narrowly approved a ballot measure last year that requires the state to reintroduce the animal on public lands in the western part of the state by the end of 2023.

Gray wolves lost their federal protected status as an endangered species this year. But they remain protected at the state level, and hunting the animals in Colorado is illegal. Penalties for violations include fines, jail time and a loss of hunting license privileges.

“These pups will have plenty of potential mates when they grow up to start their own families,” Polis said in a statement.

Gray wolves were hunted, trapped and poisoned into extermination in Colorado in the 1940s.

Officials last year confirmed the presence of a small pack of wolves in northwestern Colorado after a number of sightings since 2019. The animals were believed to have come down from Wyoming’s Yellowstone National Park.

Opponents of the reintroduction initiative said the presence of wolves in Colorado showed reintroducing the animals was unnecessary because they eventually could repopulate the state naturally.

Cattle ranchers, elk hunters, farmers and others in rural areas argue that the wolf reintroduction is bad policy driven by urban majorities along Colorado’s Front Range — and a threat to livestock and to a $1 billion hunting industry.

Wildlife advocates see reintroduction in Colorado as a vital step in restoring the wolf more quickly to habitat stretching from the Canada to the Mexico border.

Wolves were reintroduced in the Northern Rockies in the 1990s, and some 3,000 of the animals now roam portions of Montana, Wyoming, Idaho, Oregon, Washington and Northern California.

A remnant population in the western Great Lakes region has expanded to about 4,400 wolves in Michigan, Minnesota and Wisconsin.

A small population of Mexican gray wolves remains protected in the Southwest, where federal wildlife managers this week announced they had placed a record 22 captive born wolf pups into dens in the wild to be raised by surrogate packs.