HIV arrived in the US long before ‘Patient Zero’
IN THE tortuous mythology of the AIDS epidemic, one legend never seems to die: Patient Zero, aka Gaétan Dugas, a globe-trotting, sexually insatiable French Canadian f light attendant who supposedly picked up HIV in Haiti or Africa and spread it to dozens, even hundreds, of other men before his death in 1984.
Dugas was once blamed for setting off the entire US AIDS epidemic, which traumatised the nation in the 1980s and has since killed more than 500,000 Americans. The New York Post even described him with the headline The Man Who Gave Us AIDS.
But after a new genetic analysis of stored blood samples, bolstered by some intriguing historical detective work, scientists onWednesday declared him innocent.
The strain of HIV responsible for almost all AIDS cases in the United States, which was carried from Zaire to Haiti around 1967, spread from there to NewYork City around 1971, researchers concluded in the journal Nature. From New York, it spread to San Francisco around 1976.
The new analysis shows that Dugas’ own blood, sampled in 1983, contained a viral strain already infecting men in New York before he began visiting gay bars there after being hired by Air Canada in 1974.
The researchers also reported that originally Dugas was not even called Patient Zero – in an early epidemio- logical study of cases, he was designated Patient O, for “outside Southern California”, where the study began. The ambiguous circular symbol on a chart was later read as a zero, stoking the notion that blame for the epidemic could be placed on one man.
Myths like that of Patient Zero echo in prevention efforts even today, experts said. Many vulnerable groups, including young gay men and African women, fail to use protective drugs or avoid testing because they fear being stigmatised or accused of being carriers.
Reflecting on the epidemic’s early days, Dr Anthony Fauci, then a doctor treating AIDS patients and now the director of the National Institute of Allergy and Infectious Diseases, said he remembered it seeming plausible at the time that one person was responsible.
In hindsight, he added, the idea now seems absurd. “We were unaware of how widespread it was in Africa,” Fauci said. “Also, we thought, based on very little data, that it was only about two years from infection to death.”
The new data are consistent with the scenario described in 2011 in The Origins of AIDS, by Dr Jacques Pépin, an infectious disease specialist at the University of Sherbrooke in Quebec.
Relying on previous genetic research and African colonial records, Pépin showed that HIV was carried from Kinshasa to Haiti in the 1960s – most likely by one of the thousands of Haitian civil servants recruited by the United Nations to work in the former Belgian Congo after colonial rule collapsed.
The blood samples analysed in the new study were collected in 1978 and 1979 in New York and San Francisco as part of an effort to make a hepatitis B vaccine. Researchers stored almost 16,000 blood samples; nearly 7 percent of those from New York and 4 percent of those from California later turned out to be infected with HIV.
A team led by Michael Worobey, an evolutionary biologist at the University of Arizona in Tucson and lead author of the Nature paper, sequenced the genomes of the HIV found in some of those samples and compared them with viral DNA in samples collected in the early 1980s from Haitians, Dominicans and others treated in US hospitals.
Because decades spent in freezers had degraded many samples,Worobey said, his lab developed an “RNA jackhammering” technique similar to that used to reconstruct the ancient Neanderthal genome. Counting mutations allowed the researchers to“wind back the molecular clock” and see when each strain of HIV diverged from its ancestors.
Africa has a dozen HIV groups, and Haiti’s epidemic came from one of those. The New York samples all derive from one Haitian strain, and those from San Francisco are all so closely related that they probably all resulted from one person introducing one NewYork strain, Worobey said.
The symptoms that were later called AIDS were first recognised in 1981, and the legend of Patient Zero began with a 1984 study that traced the sexual contacts of 40 gay men with Kaposi’s sarcoma or other indicators of late-stage AIDS. Eight of them, half in New York and half in Southern California, had sex with an unidentified flight attendant.
Initially described as “Case 057” and then as Patient O, he reported having about 250 sexual partners a year.
Dr Harold Jaffe, who was one of the original investigators and is now the associate director for science at the Centers for Disease Control and Prevention, said the text of the original article referred to a “patient outside California”. But the chart, of which he had an early copy, was admittedly ambiguous. At the centre is the “O” or “0”, identified as the “index patient”. The other cases are numbered: “LA3” and “NY15”, for example.
The legend itself sprang from the publicity campaign for a best-selling 1987 book, And the Band Played On, by Randy Shilts, a gay San Francisco journalist who himself died of AIDS in 1994.
In a 1993 interview, Shilts said he had heard CDC investigators use the term Patient Zero and thought “Oooh, that’s catchy”. By hunting down former boyfriends of men in the 1984 study, Shilts established that the flight attendant was Dugas, who was born in Quebec but lived his last years in Vancouver, British Columbia.