不同干燥条件连翘叶茶有效成分含量比较

麻景梅 1，2，王迎春 1，2，李琛 1，2，王梦 1，2，郑倩 3，牛丽颖 1，2

1.河北中医学院，河北 石家庄 050091；2.河北省中药配方颗粒工程技术研究中心，河北 石家庄 050091；

3.石家庄市中医院，河北 石家庄 050001

摘要：目的 比较不同干燥条件下连翘叶茶中总黄酮、连翘酯苷 A 和连翘苷含量，确定连翘叶茶最佳干燥方式。方法 采用紫外分光光度法，以亚硝酸钠-三氯化铝-氢氧化钠溶液体系为显色剂，在波长 500 nm 处测定总黄酮含量；采用HPLC同时测定连翘酯苷A和连翘苷含量，色谱柱为 Diamosil C18（2）柱（4.6 mm×

250 mm，5 µm），流动相为乙腈-0.4%乙酸水溶液，梯度洗脱，检测波长 277 nm，柱温 30 ℃，流速 1.0 mL/min。结果 不同干燥条件连翘叶茶中总黄酮含量基本相同；不同干燥条件连翘叶茶中连翘酯苷 A 和连翘苷含量顺序为：通风阴干干燥＞真空干燥＞鼓风干燥。结论 不同干燥条件对连翘叶茶中总黄酮含量无明显影响，对连翘酯苷A和连翘苷的含量影响较大，通风阴干比鼓风干燥和真空干燥更有利于连翘酯苷A和连翘苷成分的保存。关键词：连翘叶茶；总黄酮；连翘酯苷A；连翘苷；紫外分光光度法；高效液相色谱法

DOI：10.3969/j.issn.1005-5304.2017.08.017

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)08-0076-04

Comparison of Effects of Different Drying Methods on Contents of Effective Components of

Forsythiae Fructus Leaves Tea MA Jing-mei1,2, WANG Ying-chun1,2, LI Chen1,2, WANG Meng1,2, ZHENG Qian3, NIU Li-ying1,2 (1. Hebei University of Chinese Medicine, Shijiazhuang 050091, China; 2. Hebei TCM Formula Granule Engineering Technology Research Center, Shijiazhuang 050091, China; 3. Shijiazhuang Traditional Chinese Medicine Hospital, Shijiazhuang 050001, China)

Abstract: Objective To compare the contents of total flavonoids, forsythoside A and phillyrin in Forsythiae Fructus leaves tea under different drying conditions; To determine the best drying method for Forsythiae Fructus leaves tea. Methods With the sodium nitrite-aluminum trichloride-sodium hydroxide solution as color reagent, total flavonoids in forsythiae Fructus leaves tea were determined by UV spectrophotometry at the wavelength of 500 nm. HPLC was used to determine the contents of forsythiaside A and phillyrin. The analysis was performed on a Diamosil

C18 (2) column (4.6 mm × 250 mm, 5 µm); the mobile phase was composed of acetonitrile and 0.4% acetic acid with gradient elution; the detection wavelength was set at 277 nm; the flow rate was 1.0 mL/min at column temperature of

30 ℃. Results The content of total flavonoids of Forsythiae Fructus leaves tea under different drying conditions was basically the same; the sequence of the contents of forsythoside A and Forsythin of Forsythiae Fructus leaves tea under different drying conditions was: ventilated drying > vacuum drying > blast drying. Conclusion Different drying conditions have no effect on the contents of total flavonoids in Forsythiae Fructus leaves tea, but have obvious effects on the contents of forsythiaside A and phillyrin. Ventilation shade is better than blast drying and vacuum drying for preverence of forsythiaside A and forsythin.

Key words: Forsythiae Fructus leaves tea; total flavonoids; forsythoside A; phillyrin; UVspectrophotometry; HPLC

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连翘叶为木犀科植物连翘 Forsythia suspensa （Thunb.）Vahl 的干燥叶，连翘的入药部位为其果实，其叶不作为药用。近年来研究发现，连翘叶化学成分

基金项目：河北省食品药品监督管理局普通项目（PT2014056）；

河北省高等学校科学技术研究重点项目（ZD2015001）

通讯作者：牛丽颖，E-mail：niuliyingyy@163.com 组成与其果实有极大相似性，且有效成分连翘酯苷A

和连翘苷的含量高于连翘果实[1-2]。现代药理研究表明，连翘叶具有抗炎、抗菌、抗衰老、保护肝脏、调

节免疫功能等作用[3]。民间有将连翘叶制成茶饮用的习惯，将连翘叶开发成茶品，对连翘资源的综合利用有重要意义。茶叶制作工艺中尤为重要的环节是干燥，常见的干燥方式主要有烘干、阴干等。为研究不