调心方对 APP/PS1 双转基因AD小鼠空间学习记忆能力和海马突触传递长时程增强的影响

王晓雯，邓海燕，王健，徐颖

上海中医药大学，上海 201203

摘要：目的 观察调心方对 APP／PS1 双转基因阿尔茨海默病（AD）小鼠空间学习记忆能力和海马突触传递长时程增强（LTP）的影响，探讨其作用机制。方法 将 54 只 3 月龄 APP/PS1 双转基因AD小鼠随机分为模型组、调心方组和阳性药组，以同月龄同性别 18 只 C57BL/6J 野生小鼠作为正常组，各给药组给予相应药物灌胃。给药12周后，进行 Morris水迷宫行为学检测和离体电生理记录。结果 Morris 水迷宫测试结果显

示，定位航行实验与模型组比较，调心方组逃避潜伏期时间明显缩短（P＜0.01）；空间探索实验与模型组比较，

调心方组小鼠穿越原平台所在象限次数和在原平台所在象限停留时间明显增加（P＜0.05，P＜0.01）。在离体

海马电生理诱导中，与模型组比较，调心方组海马兴奋性突触后电位斜率明显增强（P＜0.01），海马双脉冲易

化无明显差异（P＞0.05）。结论 调心方可有效改善 APP/PS1 双转基因 AD 小鼠空间学习记忆能力和神经突触可塑性，具有认知保护作用。

关键词：调心方；Morris水迷宫；海马突触传递长时程增强；阿尔茨海默病；学习记忆能力；小鼠

DOI：10.3969/j.issn.1005-5304.2017.10.010

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)10-0040-04

Effects of Tiaoxin Formula on Spatial Learning and Memory Ability and LTP in Hippocampus of APP/PS1 Double Transgenic Mice with Alzheimer Disease

WANG Xiao-wen, DENG Hai-yan, WANG Jian, XU Ying (Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

Abstract: Objective To investigate the effects of Tiaoxin Formula on spatial learning and memory ability and long-term potentation (LTP) in the hippocampus of APP/PS1 transgenic mice with Alzheimer disease; To discuss its mechanism of action. Methods Totally 54 three-month-old APP/PS1 transgenic mice were randomly divided into model control group, Tiaoxin Formula group and positive control group, with 18 mice in each group. Another 18 three-month-old C57BL/6J wild mice were chosen as normal control group. All administration groups receive relevant medicine. 12 weeks later, Morris water maze test was used to test behavior and in vitro electrophysiology record. Results The Morris water maze test showed that in place navigation test, compared with the model control group, escape latency time in Tiaoxin Formula group was significantly reduced (P<0.01). In the space experiments, compared with the model control group, the number of crossing the platform quadrant and the time staying at the platform quadrant in Tiaoxin Formula group were significantly increased (P<0.05, P<0.01). In vitro hippocampal electrophysiological induction, compared with the model control group, fEPSP slope in the hippocampus in Tiaoxin Formula group were significantly increased (P<0.01), the PPF had no significant difference (P>0.05). Conclusion Tiaoxin Formula can improve the spatial learning and memory ability and LTP of APP/PS1 transgenic mice with Alzheimer disease, thus can realize cognitive protection effects.

Key words: Tiaoxin Formula; Morris water maze; LTP in hippocampus; Alzheimer disease; learning and memory ability; mice 阿尔茨海默病（Alzheimer disease，AD）是常见

基金项目：上海市自然科学基金（13ZR1439700）

通讯作者：王健，E-mail：wangjiantcm@126.com；徐颖，E-mail：

ying6122003@aliyun.com 于老年人群的一种不可逆转的中枢神经退行性疾病，是老年痴呆最常见的类型。AD 早期症状主要有渐进性记忆的减退、认知功能下降等，后期常出现抑郁、焦虑、幻觉等异常精神症状，导致患者生活能力下降及社会参与度降低。AD属中医学“呆证”“癫证”“郁