止嗽散加减方对慢性阻塞性肺疾病寒燥模型大鼠表皮生长因子受体及中性粒细胞弹性蛋白酶的影响

高振，王晶，姜敏，李争，徐丹，哈木拉提•吾甫尔，荆晶，李风森

新疆医科大学附属中医医院国家中医临床研究基地，新疆 乌鲁木齐 830000

摘要：目的 观察止嗽散加减方对慢性阻塞性肺疾病（COPD）寒燥模型大鼠表皮生长因子受体（EGFR）及中性粒细胞弹性蛋白酶（NE）的影响。方法 利用气管滴注弹性蛋白酶结合烟熏90 d建立 COPD 模型，在此基础上予以寒燥应激90 d建立 COPD寒燥模型。将COPD寒燥模型大鼠随机分为寒燥组和中药组，中药组连续干预7d。第97日处死大鼠，观察其肺组织病理形态。RT-qPCR和 Western blot分别检测肺组织EGFR mRNA和蛋白表达，ELISA检测血清和肺泡灌洗液NE含量。结果 COPD组、寒燥组、中药组肺组织EGFR mRNA和蛋白表达较对照组均升高，中药组肺组织 EGFR 蛋白表达较 COPD 组和寒燥组均降低；COPD 组、寒燥组和中药组血清和肺泡灌洗液NE含量较对照组升高，寒燥组肺泡灌洗液NE含量高于 COPD组，中药组肺泡灌洗液NE含量低于寒燥组。结论 止嗽散加减方可能通过降低寒燥COPD模型大鼠肺组织EGFR和NE的水平，达到治疗 COPD的目的。关键词：慢性阻塞性肺疾病；寒燥；止嗽散加减方；表皮生长因子受体；中性粒细胞弹性蛋白酶

DOI：10.3969/j.issn.1005-5304.2017.10.013

中图分类号：R285.5 文献标识码：A 文章编号：1005-5304(2017)10-0053-04

Effects of Modified Zhisou Power on Epidermal Growth Factor Receptor and Neutrophil Elastase in Rats with Cold-Dryness Chronic Obstructive Pulmonary Disease

GAO Zhen, WANG Jing, JIANG Min, LI Zheng, XU Dan, Halmurat UPUR, JING Jing, LI Feng-sen (National Clinical Research Base of Traditional Chinese Medicine, Traditional Chinese Medicine Hospital Affiliated to Xinjiang Medical University, Urumqi 830000, China)

Abstract: Objective To reveal the effects of cold-dryness and modified Zhisou Power on epidermal growth factor receptor (EGFR) and neutrophil elastase (NE) in rats with COPD. Methods COPD model was established with an elastase dose into the trachea combined with exposure to smoking for 90 d; cold-dryness COPD group was further developed by exposure to a cold, dry environment for 90 d. After 90 days, cold-dryness COPD rats was divided into cold-dryness group and Zhisou Power intervention group (treated with modified Zhisou Power for 7 days). On the 97th day, all rats were killed. Pathological changes in lungs were observed. mRNA and protein expression of EGFR were measured by RT-qPCR and Western blot, and the amount of NE in serum and BALF were examined by ELISA. Results EGFR mRNA and protein expression were significantly higher in COPD group, cold-dryness group and Zhisou Power intervention group than in control group. EGFR was significantly lower in Zhisou Power intervention group than in COPD group and cold-dryness group. NE was significantly higher in serum and BALF in COPD group, cold-dryness group and Zhisou Power intervention group than in control group. NE in BALF was significantly higher in cold-dryness group than in COPD group. NE in BALF was significantly lower in Zhisou Power intervention group than in cold-dryness group. Conclusion Modified Zhisou Power can down-regulate the expression of EGFR and the mount of NE in cold-dryness COPD rats to treat COPD.

Key words: COPD; cold-dryness; modified Zhisou Power; epithelial growth factor receptor; neutrophil elastase 新疆地处中国西北部，为世界上离海洋最远的地

基金项目：新疆维吾尔自治区自然科学基金（2015211C138）

通讯作者：李风森，E-mail：fengsen602@163.com 区，气候干燥寒冷。新疆慢性阻塞性肺疾病（chronic obstructive pulmonary disease，COPD）在中医证型、证素、症状等中医特点上与内地有别，表现出寒、燥的临床特点[1]。流行病学调查显示，西北寒燥证是新