基于多指标分析甘肃产掌叶大黄质量

陆国弟1，李成义 1，王惠珍 1，杨扶德 1，王明伟 1，沈俊忠 2

1.甘肃中医药大学药学院，甘肃 兰州 730000；2.宕昌县科技局，甘肃 陇南 748500

摘要：目的 采用指纹图谱与一测多评相结合的方法测定掌叶大黄5种指标性成分，分析比较甘肃不同产区、不同生长年限掌叶大黄质量，确定其最适种植区和最佳发展区。方法 采用药典方法测定掌叶大黄含水量、总灰分、水溶性浸出物，HPLC 测定 5种指标性成分，指纹图谱与一测多评相结合评价 15 批掌叶大黄质量。结果 15批掌叶大黄的含水量均小于15%，总灰分均小于10%，水溶性浸出物均大于25%，符合药典标准；

15批样品指纹图谱标定共有峰23个，其中已确定成分芦荟大黄素线性范围0.012 2～0.734 4 µg（r＝0.999 9）、大黄酸线性范围 0.007 14～5.712 0 µg（r＝0.999 9）、大黄素线性范围 0.008 8～0.704 0 µg（r＝1.000 0）、大黄酚线性范围 0.122 4～3.672 0 µg（r＝0.999 9）、大黄素甲醚线性范围 0.014 8～5.920 0 µg（r＝0.999 7）；采用相对校正因子计算的含量值与实测值之间无显著差异，建立的相对校正因子重现性良好。甘肃产掌叶大黄质量在

不同产区、不同年限间存在显著差异（P＜0.05），其中礼县上萍乡、宕昌南河乡茹树村所产2年生掌叶大黄质量最好，宕昌地区掌叶大黄3年生优于2年生。结论 所建立的指纹图谱和一测多评方法准确、可行，可用于甘肃掌叶大黄质量比较。各产区中，以礼县和宕昌地区适宜种植及发展大黄产业，甘肃礼县和宕昌地区2年生以上掌叶大黄适合药用。

关键词：掌叶大黄；指纹图谱；一测多评法；质量比较

DOI：10.3969/j.issn.1005-5304.2017.10.014

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)10-0057-07

Analysis on Quality of Rheum Palmatum L. from Gansu Province Based on Multicriteria Method

LU Guo-di1, LI Cheng-yi1, WANG Hui-zhen1, YANG Fu-de1, WANG Ming-wei1, SHEN Jun-zhong2

(1. Gansu University of Chinese Medicine, Lanzhou 730000, China; 2. Tanchang Science and Technology Bureau, Longnan 748500, China)

Abstract: Objective To compare the quality of Rheum palmatum L. in different areas and ages in Gansu Province by comparing five components in Rheum palmatum L. through fingerprints and QAMS (quantitative analysis of multi-components) methods; To determine the most suitable growing areas and the best development areas in Gansu Province. Methods The water content, total ashes, and water-soluble extract content in 15 batches of Rheum palmatum L. from different areas and ages in Gansu Province were determined according to the approaches listed in the Chinese Pharmacopeia. The contents of five index components were determined through HPLC and the quality of

15 batches of Rheum palmatum L were evaluated by fingerprint and QAMS method. Results The results from 15 batches of Rheum palmatum L. showed that the water content was less than 15%, total ashes less than 10%, and water-soluble extract content less than 25%, conforming to Pharmacopoeia. There were 23 common peaks in the fingerprints of 15 batches of Rheum palmatum L.. Five of them were identified as aloe-emodin, rhein, emodin, chrysophanol and physcion, which showed good linear relationship in the range of 0.012 2–0.734 4 µg (r=0.999 9),

0.007 14–5.712 0 µg (r=0.999 9), 0.008 8–0.704 0 µg (r=1.000 0), 0.122 4–3.672 0 µg (r=0.999 9) and 0.014 8–

5.920 0 µg (r=0.999 7), respectively. There was no significant difference between the calculated value and the measured value by using the relative correction factor (RCF), and the reproducibility of RCF was good. The quality of

基金项目：甘肃中医药大学中青年科研基金项目（2305016601）

通讯作者：王惠珍，E-mail：whz1974828@163.com