HPLC同时测定参积护胃颗粒中积雪草苷、延胡索乙素、柴胡皂苷d含量

姜正华，李劲平，石丽颖，谢保平

中南大学湘雅药学院，湖南 长沙 410013

摘要：目的 建立参积护胃颗粒中积雪草苷、延胡索乙素、柴胡皂苷 d 含量测定的 HPLC 分析方法。方法 采用广州研创C18 色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈（A）-磷酸盐缓冲液（含磷酸二氢钾 8.34‰、磷酸氢二钾 0.87‰），梯度洗脱（0～15 min，20%A；15～30 min，20%→40%A；30～42 min，

40%A；42～45 min，40%→48%A；45～50 min，48%A；50～70 min，48%→50%A），流速 1.0 mL/min，检测波长 210 nm，柱温 30 ℃。结果 积雪草苷、延胡索乙素、柴胡皂苷d分别在 0.173～2.770 μg（r＝0.999 9）、

0.021～1.320 μg（r＝0.999 2）、0.151～9.660 μg（r＝0.999 3）范围内呈良好线性关系。积雪草苷、延胡索乙素、柴胡皂苷d平均加样回收率分别为 96.25%、97.02%、97.84%，RSD 分别为 2.31%、4.51%、1.87%。结论 该含量测定方法简便、分离效果好、专属性强，能同时测定参积护胃颗粒中积雪草苷、延胡索乙素、柴胡皂苷d的含量，为参积护胃颗粒质量标准的完善提供依据。关键词：参积护胃颗粒；积雪草苷；延胡索乙素；柴胡皂苷d；高效液相色谱法；质量控制

DOI：10.3969/j.issn.1005-5304.2017.10.015

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)10-0064-04

Simultaneous Determination of Contents of Asiaticoside, Saikosaponin d in Shenji Huwei Granules by HPLC Tetrahydropulmatine and

JIANG Zheng-hua, LI Jin-ping, SHI Li-ying, XIE Bao-ping (Xiangya School of Pharmaceutical Science, Central South University, Changsha 410013, China)

Abstract: Objective To develop an HPLC method for simultaneous determination of contents of asiaticoside, tetrahydropulmatine and saikosaponin d in Shenji Huwei Granules. Methods The analysis was performed on a R&C

C18 column (250 mm × 4.6 mm, 5 μm) by using the mobile phase of acetonitrile (A) - phosphate buffer (which used potassium dihydrogen phosphate 8.34 g, potassium phosphate 0.87 g dissolved by 1000 mL water) with gradient elution (0–15 min, 20%A; 15–30 min, 20%→40%A; 30–42 min, 40%A; 42–45 min, 40%→48%A; 45–50 min,

48%A; 50–70 min, 48%→50%A). The flow rate was 1.0 mL/min; the detection wavelength was set at 210 nm; the column temperature was maintained at 30 ℃. Results Asiaticoside, tetrahydropulmatine and saikosaponin d were in the linear ranges among 0.173–2.770 μg (r=0.999 9), 0.021–1.320 μg (r=0.999 2), 0.151–9.660 μg (r=0.999 3), respectively. The average recovery rates of asiaticoside, tetrahydropulmatine, saikosaponin d were 96.25%, 97.02%, and 97.84%, respectively, and RSD were 2.31%, 4.51%, 1.87%, respectively. Conclusion This method is simple, with good separation effect and strong specificity, and can be used for simultaneous determination of contents of asiaticoside, tetrahydropulmatine and saikosaponin d in Shenji Huwei Granules, which provides references for perfection of quality control of Shenji Huwei Granules.

Key words: Shenji Huwei Granules; asiaticoside; tetrahydropulmatine; saikosaponin d; HPLC; quality control

参积护胃颗粒是由湖南中医药大学第一附属医院莫新民教授的临床经验方研制而成的颗粒剂，由党参、延胡索、柴胡等8味药组成，具有健脾疏肝、活

基金项目：湖南省科技计划（2009SK3190）

通讯作者：李劲平，E-mail：pjingli@163.com 血止痛功效，主要治疗胃溃疡、慢性胃炎等消化系统疾病。本课题组前期研究显示，参积护胃颗粒具有抗

实验性胃溃疡的作用[1]。现代药理研究表明，党参中的党参炔苷及党参提取物对胃黏膜损伤有保护作

用[2]；柴胡中的柴胡总皂苷对无水乙醇致大鼠胃黏膜

损伤具有明显的抑制作用[3-5]，两者为方中君药。延