HPLC同时测定唐古特大黄地上部分6种化学成分含量

陈丽萍，李茂星，马幸福

兰州军区兰州总医院药材科，全军高原环境损伤防治重点实验室，甘肃 兰州 730050摘要：目的 建立 HPLC 同时测定唐古特大黄地上部分中大黄酚、大黄素、大黄素甲醚、芦荟大黄素、番泻苷A和番泻苷B共 6种成分含量的方法。方法 采用 Agilent ZORBAX SB-C18 色谱柱（250 mm×4.6 mm，

5 —m），流动相为甲醇（A）-0.1%磷酸水溶液（B），梯度洗脱（0 min，70%B；5 min，65%B；8～16 min，60%B；

18～23 min，55%B；25～30 min，45%B；32～39 min，35%B；49～57 min，24%B；65～72 min，20%B）；流速 1.0 mL/min，检测波长 254 nm，柱温为室温，按外标法定量分析。结果 大黄素、大黄酚、大黄素甲醚、芦荟大黄素、番泻苷 A、番泻苷 B 在一定范围内与峰面积积分值线性关系良好（r≥0.999 5），方法的精密度RSD 为 0.75%～1.17%，重复性 RSD 为 0.99%～2.06%，稳定性 RSD 为 0.97~1.76%，平均加样回收率为 99.7%～

100.4%。唐古特大黄根和地上部分 6 种化学成分含量存在差异。结论 所建立的方法能同时测定唐古特大黄地上部分6种成分的含量，可作为大黄地上部分化学成分含量测定的参考方法。关键词：唐古特大黄；大黄素；大黄酚；大黄素甲醚；芦荟大黄素；番泻苷A；番泻苷B；高效液相色谱法

DOI：10.3969/j.issn.1005-5304.2017.10.018

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)10-0076-05

Simultaneous Determination of Contents of 6 Components tanguticum Maxim.ex Balf by HPLC Method in Aerial Part of Rheum

CHEN Li-ping, LI Mao-xing, MA Xing-fu (Department of Pharmacy, Lanzhou General Hospital of PLA, Key Laboratory of the Prevention and Cure for the Plateau Environment Damage, PLA, Lanzhou 730050, China)

Abstract: Objective To develop an HPLC method to determine the contents of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B simultaneously in the aerial part of Rheum tanguticum Maxim.ex Balf. Methods The analysis was achieved with an Agilent ZORBAX SB-C18 analytic column (250 mm × 4.6 mm, 5 μm) by gradient elution of methanol-0.1% phosphoric acid in water gradient elution system (0 min, 70% B; 5 min, 65% B; 8–16 min, 60% B; 18–23 min, 55% B; 25–30 min, 45% B; 32–39 min, 35% B; 49–57 min, 24% B; 65–72 min, 20% B). The flow rate was 1.0 mL/min; detection wavelength was 254 nm; the column temperature was room temperature; the quantification used external standard method. Results The peak areas and concentrations of chrysophanol, emodin, physcion, aloe-emodin, sennoside A, and sennoside B showed good linear relationship within a certain concentration range (r≥0.999 5); the RSD of precision was 0.75%–1.17%; the RSD of repeatability was 0.99%–2.06%; the RSD of stability was 0.97%–1.76%; the average recoveries were 99.7%–100.4%. The results showed that there were differences in content between the root and aerial part of Rheum tanguticum Maxim.ex Balf. Conclusion HPLC method for simultaneous determination of contents of 6 contents of the aerial part of Rheum tanguticum Maxim.ex Balf can be used as the references for quality control.

Key words: Rheum tanguticum Maxim.ex Balf; chrysophanol; emodin; physcion; aloe-emodin; sennoside A; sennoside B; HPLC 大黄被《神农本草经》列为中品，以干燥根和根茎入药，《中华人民共和国药典》规定的原植物为蓼

基金项目：

国家自然科学基金（ 30672643、30472186、

30772773）；甘肃省自然科学基金（3ZS051-A25-078）

通讯作者：李茂星，E-mail：limaox2005@aliyun.com 科植物唐古特大黄 Rheum tanguticum Maxim. ex Balf.、掌叶大黄 Rheum palmatum L.或药用大黄Rheum officinale Baill.[1]，属多年生宿根草本植物。唐古特大黄 Rheum tanguticum Maxim.ex Balf.又称“鸡爪大黄”，主产于甘肃、青海、西藏等海拔2500 m以上的高寒、高海拔地区[2]，在我国已有多年的栽培历史。据文献