HPLC指纹图谱结合主成分分析对不同产地头顶一颗珠质量的评价

陈洁 1，2，陈文茜 1，郭文鼎 1，雷滔 1，欧阳俊摇 1，2，赵晖 1，2，邹海艳 1，2

1.首都医科大学中医药学院，北京 100069；2.中医络病研究北京市重点实验室，北京 100069摘要：目的 通过 HPLC指纹图谱结合主成分分析（PCA）对不同产地头顶一颗珠药材的质量进行评价，为其质量控制提供依据。方法 采用 Hibar C18 色谱柱（4.6 mm×250 mm，5 µm），乙腈-水为流动相进行梯度洗脱，流速为 1.0 mL/min，检测波长为 203 nm，柱温为 30 ℃，利用相似度评价软件和PCA 对 14批头顶一颗珠药材检测结果进行分析比较。结果 HPLC指纹图谱的方法学考察符合相关要求，共标定了头顶一颗珠药材HPLC指纹图谱的15 个共有峰，14批药材的相似度为 0.389～0.979。PCA 结果显示前3个主成分的累积方差贡献率为 87.674%，S2 样品综合得分最高（4.926）、质量最好。结论 所建立的 HPLC 指纹图谱结合 PCA 可以客观、有效地评价不同产地头顶一颗珠药材的质量。关键词：头顶一颗珠；指纹图谱；高效液相色谱法；主成分分析；相似度分析

DOI：10.3969/j.issn.1005-5304.2017.11.014

中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2017)11-0058-05

Evaluation of Quality Coherence of Trillium tschonoskii Maxim. from Different Producing Areas Based on HPLC Fingerprint and PCA

CHEN Jie1,2, CHEN Wen-xi1, GUO Wen-ding1, LEI Tao1, OUYANG Jun-yao1,2, ZHAO Hui1,2, ZOU Hai-yan1,2 (1. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China; 2. Beijing Key Lab of TCM Collateral Disease Theory Research, Beijing 100069, China)

Abstract: Objective To evaluate the quality coherence of Trillium tschonoskii Maxim. from different producing areas by HPLC fingerprint and PCA; To provide a method for quality control. Methods Samples were separated by Hibar C18 (4.6 mm × 250 mm, 5 µm) with acetonitrile-water as gradient mobile phase at the flow rate of 1.0 mL/min. The wavelength was 203 nm and the temperature was 30 ℃. Chromatographic Fingerprint Similarity Evaluation System and PCA were used to analyze the data. Results The results of method validation of HPLC fingerprint met technical standards. 15 common peaks was verified and the similarities of 14 batches of Trillium tschonoskii Maxim. from different producing areas were among 0.389–0.979. 3 principal components with the characteristic root cumulative contribution rate reaching 87.674% were screened out by PCA results. The composite score of S2 was the highest (4.926), and the quality was the best. Conclusion The application of HPLC combined with PCA can objectively and effectively evaluate the quality difference of Trillium tschonoskii Maxim. from different producing areas.

Key words: Trillium tschonoskii Maxim.; fingerprint; HPLC; principal component analysis; similarity analysis 头顶一颗珠，又名芋儿七，为百合科延龄草属植物延龄草 Trillium tschonoskii Maxim.的干燥根和根 茎，主要分布于我国陕、川、鄂、湘等地，是土家族、苗族等少数民族常用药材。据《土家族药物志》记载，头顶一颗珠性平，味甘，有小毒，功能镇静止痛、散瘀疗伤、活血调经和止血[1]，是土家族四大神药（文王一支笔、江边一碗水、七叶一枝花、头顶一颗珠）之一。现代研究表明，头顶一颗珠具有抗炎、镇痛、抗肿瘤、促凝血、增强心脏功能、免疫调节、抗氧化、