Study on Purification Process of Total Flavonoids from Dragon’s Blood

ZHOU Yunlong, PENG Shuang, LI Jiang, LI Zhengyan, YU Xiaoling, CHEN Lingyun

ZHOU Yunlong1,2, PENG Shuang1, LI Jiang1, LI Zhengyan3, YU Xiaoling1,3, CHEN Lingyun1

1. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China;

2. Yunnan Institute of Materia Medica, Kunming 650111, China;

3. Kunming Hospital of Traditional Chinese Medicine, Kunming 650051, China

Abstract: Objective To establish a purification process for total flavonoids from Dragon’s blood. Methods The adsorption rate and desorption rate of Loureirin A and Loureirin B were used to evaluate the purification process. The macroporous adsorption resin was used to purify the total flavonoids of Dragon’s blood, and the best process was determined. Results The optimal process for the purification of total flavonoids from Dragon’s blood by macroporous adsorption resin was as follows: D101 macroporous resin was selected, and the ethyl acetate extract of Dragon’s blood was dissolved in 0.3% NaOH solution. The concentration of the sample was 12 mg/mL. The loading amount was

2 BV. The loading volume was 1.0 BV/h. The impurities were eluted with purified water until the effluent was neutral, and then eluted with 80% ethanol 5 BV to obtain the total flavonoids of Dragon’s blood. The contents of Loureirin A and Loureirin B was increased by 3 times compared with the materia medica. Conclusion D101 macroporous resin can be used in the purification process of total flavonoids from Dragon’s blood. The process is stable and reliable.

Keywords: Dragon’s blood; total flavonoids; macroporous resin; purification process