Unlocking the secrets of the microbiome
MODERN technology is making it possible for medical scientists to analyze inhabitants of our innards that most people probably would rather not know about. But the resulting information could one day save your health or even your life.
I’m referring to the trillions of bacteria, viruses and fungi that inhabit virtually every body part, including those tissues once thought to be sterile. Together, they make up the human microbiome and represent what is perhaps the most promising yet challenging task of modern medicine: determining the normal microscopic inhabitants of every organ and knowing how to restore the proper balance of organisms when it is disrupted.
Proof of principle, as scientists call it, has already been established for a sometimes devastating intestinal infection by the bacterium Clostridium difficile. This infection, popularly called C. diff, often occurs when potent antibiotics wipe out the normal bacterial inhabitants of the gut that otherwise keep it in check.
When all else fails to clear up a recurrent C. diff infection, the Food and Drug Administration has approved treatment with a fecal transplant from a healthy gut presumed to contain bacteria that can suppress C. diff activity. The treatment is highly effective, with a cure rate in excess of 90 percent.
Under the auspices of the National Institutes of Health, a large team of scientists is engaged in creating a “normal” microbiological road map for the following tissues: gastrointestinal tract, oral cavity, skin, airways, urogenital tract, blood and eye. The effort, called the Human Microbiome Project, takes advantage of new technology that can rapidly analyze large samples of genetic material, making it possible to identify the organisms present in these tissues.
Depending on the body site, anywhere from 20-60 percent of the organisms that make up the microbiota cannot be cultured and identified with the older, traditional techniques used by microbiologists.
If the institutes’ five-year project succeeds in defining changes in the microbiome that are associated with disease, it has the potential to transform medicine, assuming ways can be found to correct microbial distortions in the affected tissues.
Previous studies have already found differences in the gut microbiota of lean and obese adults. There is also evidence that the typical high-calorie U.S. diet rich in sugar, meats and processed foods may adversely affect the balance of microbes in the gut and foster the extraction and absorption of excess calories from food.
A diet more heavily based on plants may result in a microbiome containing a wider range of healthful organisms. In studies, mice that had a microbiota preconditioned by the typical U.S. diet did not respond as healthfully to a plant-based diet.
Compared to lean mice, obese mice have a 50 percent reduction in organisms called Bacteroidetes and a proportional increase in Firmicutes, and lean mice get fat when given fecal transplants from obese mice. A similar shift has been observed in people, and the distorted ratio of organisms was shown to reverse in people who lose weight after bariatric surgery.
There is also evidence that microbes residing in the gut can affect distant sites through their influence on a person’s immune responses. This indirect action has been suggested as a possible mechanism behind rheumatoid arthritis. In mice, certain bacteria in the gut have been shown to foster production of antibodies that attack the joints, resulting in the joint destruction typical of rheumatoid arthritis.
Studies have suggested a role of the gut microbiota in the risk of developing neuropsychiatric illnesses like schizophrenia. Researchers have suggested that in genetically susceptible people, altered microbes in the gut may disrupt the blood-brain barrier, leading to the production of antibodies that impede normal brain development.