Pittsburgh Post-Gazette

Institute aims to restore lost chestnut

American species lost to root rot, blight in 1990s

- By John Hayes

Pittsburgh Post-Gazette

The American chestnut might get a do-over. With a significan­t new round of grants, The American Chestnut Foundation, based in North Carolina, plans to develop ways to predict chestnut trees’ resistance to diseases that cause chestnut blight and root rot.

This method, known as “genomic selection,” is expected to accelerate selection of the most disease resistant trees from the groups breeding program.

“If we can develop genomic tools to restore American chestnut, we can do this with almost any forest tree species imperiled by the introducti­on of invasive pathogens” said project partner Jeremy Schmutz, director of the American chestnut genome project at HudsonAlph­a Institute for Biotechnol­ogy, in a statement. The institute is a nonprofit genomics and genetics research institute in Alabama.

Chestnut blight arrived from Asia in the early 1900s and quickly reduced an estimated 4 billion American chestnut trees to root sprouts. Phytophtho­ra root rot eradicated American chestnut from forests in the southeaste­rn Piedmont region prior to the introducti­on of chestnut blight.

The loss of American chestnut impacted other species, significan­tly reducing the population of bear, Eastern wild turkey and pollinator­s in forests of the eastern United States.

For some 30 years, the Chestnut Foundation has been attempting to breed resistance into American chestnut from Chinese chestnut, which is resistant to these diseases. The major bottleneck in the foundation’s breeding program has been in screening the thousands of trees needed to combine the right mixture of blight-resistance and American chestnut traits.

The first step in assessing a tree’s resistance, according THIS WEEK: Although muskellung­e are native to western Pennsylvna­nia, they are cultured and stocked by the state Fish and Boat Commission. Should more muskies be stocked in the regions lakes and rivers? • Yes • No to a media release from The American Chestnut Foundation, is to artificial­ly inoculate it with the fungus that causes chestnut blight. After the most susceptibl­e trees are culled, additional selections are made by screening descendent­s of the remaining trees for disease resistance. Using this method at the current rate of seed production, it would take more than 30 years to complete selection.

Genomic selection would reduce the time necessary to complete the selection of disease-resistant parents to five years. Genomic selection doesn’t create mutant nuts. Using high quality reference genome sequences for American and Chinese chestnut species, it sequences individual genes involved in disease resistance.

Researcher­s want to know why the Chinese and American chestnut species differ so much in their resistance to blight and root rot. Collaborat­ors at Pennsylvan­ia State University are assembling a reference genome sequence for Chinese chestnut. With the recently awarded funding, collaborat­ors at HudsonAlph­a intend to lead a parallel effort to assemble a reference genome sequence for American chestnut.

“Restoring a species is a hopeful and ambitious undertakin­g,” said said American Chestnut Foundation president and CEO Lisa Thomson, in a statement. “We are thankful these private family foundation­s have invested in a plan for reintroduc­tion of a resistant American chestnut tree, once one of the most important hardwood trees in the Eastern forests.”

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