USA TODAY US Edition

CRISPR tech used to develop 5-minute virus test

- Ken Alltucker

Coronaviru­s tests performed in labs are the gold standard for accuracy, and antigen tests are a fast and inexpensiv­e alternativ­e.

But backers of a third type of test, developed by a Nobel Prize winner using cutting-edge CRISPR technology, say it has the potential to be all three: rapid, accurate and inexpensiv­e.

Although these gene-editing technology tests are still being developed and won’t be ready in the United States this year as the weather cools and demand surges, research groups recently published papers describing them as an appealing alternativ­e as testing shortages persist amid the COVID-19 pandemic.

Dr. Jennifer Doudna, a University of California-Berkeley researcher whose pioneering work in CRISPR earned a share of this year’s Nobel Prize in chemistry, said the test can be done quickly and doesn’t require a lab.

“We have a ways to go before CRISPRbase­d diagnostic­s reach widespread use, but I believe we’ll see an impact during the current pandemic,” Doudna said. “Because it is simple to adjust these tests to detect other targets, the platform we’re developing now is laying the groundwork to deploy CRISPR for rapid diagnosis during future outbreaks.”

CRISPR, or clustered regularly interspace­d short palindromi­c repeats, is a gene-editing technology studied for a wide range of uses from cancer and sickle cell disease treatments to improved food production.

In 2016, Doudna’s lab developed a way to detect RNA using the technology. Her lab collaborat­ed with Dr. Melanie Ott of San Francisco-based Gladstone Institutes to develop an HIV test, but when the pandemic hit, the researcher­s focused on developing a coronaviru­s test.

The test recognizes a sequence of RNA in SARS-CoV-2, the coronaviru­s that causes COVID-19.

In an Oct. 12 publicatio­n, researcher­s reported the test yielded results in five minutes and correctly identified five samples from patients with the coronaviru­s. When used with a mobile phone to detect signals generated by the test, the technology could provide a fast,

low-cost test outside a laboratory, researcher­s said in the paper, which was not peer-reviewed.

Massachuse­tts Institute of Technology scientists also are honing a CRISPR-based test that can be used outside a lab. In a New England Journal of Medicine letter published last month, researcher­s said the test was evaluated at a University of Washington lab using 202 samples with the coronaviru­s and 200 without. The test correctly identified 93.1% of positive samples. The test also had 98.5% specificit­y, which means it rarely reported false positives.

Feluda, a paper-based CRISPR test named after a fictional India detective, has been cleared by that nation’s drug regulators for commercial launch. But it’s unclear how the Indian conglomera­te Tata Group plans to deploy the test in India, which trails only the United States with nearly 7.7 million cases, according to data from Johns Hopkins University.

Testing that is fast and accurate

Gigi Gronvall, a senior scholar at the Johns Hopkins Center for Health Security, said she expects more labs will explore tests using this technology. She called it “extremely promising” because people can use the tests outside the lab.

“That has been a big challenge with testing, generally,” Gronvall said. “People need to have their results pretty quickly otherwise they keep going about their day and they might be infectious and not stop having contact with other people.”

The south San Francisco-based biotechnol­ogy firm Mammoth Bioscience­s, co-founded by Doudna, is working to further develop its test and make it available to labs and medical providers. The company received a National Institutes of Health grant to accelerate developmen­t.

Mammoth CEO Trevor Martin said the company’s goal is to make a test that is fast and accurate.

“Right now you have to make this choice: Do you want something that is simple and fast or something that has the highest accuracy?” Martin said.

The most accurate lab tests are molecular-based polymerase chain reaction, or PCR, which amplify a small amount of genetic material from a nasal swab sample. Labs are limited in the number of PCR tests they can run because of periodic shortages of chemical reagents and other testing materials. In July, when demand outpaced labs’ ability to perform tests, consumers whose tests were routed to major labs routinely waited a week or longer for results.

Antigen tests, which detect proteins of the coronaviru­s, can produce results in 15 minutes but are considered less sensitive than lab tests.

Martin said CRISPR-based tests offer “a technology that is very simple, very fast yet extremely accurate.”

Scaling up to be cheap and accessible

Beyond the technology, test developers are trying to solve another challenge: designing a test that is easy to use and inexpensiv­e.

Dr. Feng Zhang, an MIT biochemist and core member of the Broad Institute whose lab is developing the StopCOVID test, said his team wants to make a low-cost device that can work with a disposable cartridge. Collaborat­ors want a test easy enough for consumers to use at home by taking their own nasal swab.

“The challenge is to work out how to scale up the manufactur­ing so that we can get the cost to be as low as possible,” Zhang said.

He said his collaborat­ors are working to bring the test to market as soon as possible. A version of the test already is being used by a Thailand hospital to screen patients.

Mammoth’s Martin said designing a test for wide use is “where a lot of things stumble.”

“It’s really nice to have a new technology, but you need to get it to the places where it’s needed,” he said. “That’s something we’ve been working on very diligently.”

Dr. Sophia L. Yohe, director of the University of Minnesota’s Molecular Diagnostic­s Laboratory, said it’s unknown whether test developers will be able to streamline steps to allow a large number of tests.

Some early versions of CRISPR tests required samples to be extracted and amplified, similar to a labbased PCR tests, said Yohe, chair of the College of American Pathologis­ts Personaliz­ed Health Care Committee.

“If you can do only one test at a time, even if it only takes 30 minutes, suddenly doing a thousand tests takes a long time,” she said. “So scalabilit­y, I think, is an issue that has to be considered if you are looking at doing high volumes of testing.”

 ?? SUSAN WALSH/AP ?? Dr. Jennifer Doudna said, “We have a ways to go before CRISPR-based diagnostic­s reach widespread use, but I believe we’ll see an impact during the current pandemic.”
SUSAN WALSH/AP Dr. Jennifer Doudna said, “We have a ways to go before CRISPR-based diagnostic­s reach widespread use, but I believe we’ll see an impact during the current pandemic.”

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